Protein Info for PFLU_RS23100 in Pseudomonas fluorescens SBW25

Annotation: ribonuclease E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1075 TIGR00757: ribonuclease, Rne/Rng family" amino acids 29 to 438 (410 residues), 554.8 bits, see alignment E=6.3e-171 PF00575: S1" amino acids 52 to 131 (80 residues), 47.3 bits, see alignment 3.2e-16 PF10150: RNase_E_G" amino acids 135 to 404 (270 residues), 337.4 bits, see alignment E=9.7e-105 PF20833: RNase_E_G_Thio" amino acids 416 to 500 (85 residues), 111.9 bits, see alignment E=1.9e-36

Best Hits

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 83% identity to pfl:PFL_1787)

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0P3 at UniProt or InterPro

Protein Sequence (1075 amino acids)

>PFLU_RS23100 ribonuclease E (Pseudomonas fluorescens SBW25)
MCAERRFRQPGNRRYNMKRMLINATQPEELRVALVDGQRLYDLDIESGAREQKKANIYKG
RITRIEPSLEAAFVDFGSERHGFLPLKEISREYFKKAPEGRVNIKDVLSEGQEVIVQVEK
EERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLIAPADMGL
IVRTAGLGRSSEEMQWDLDYLLQLWTAIKEASLDRSAPFLIYQESNVIIRAIRDYLRQDI
GEVLIDSVEAQDEALTFIRQVMPQYASKIKLYEDSVPLFNRFQIESQIETAFQRVVELPS
GGSIVIDPTEALVSIDINSARATKGSDIEETALQTNLEAAEEIARQLRLRDIGGLIVIDF
IDMTPAKNQRAVEEKVRECLEADRARVQVGRISRFGLLEMSRQRLRPSLGESSGIVCPRC
NGTGIIRDVESLSLAILRLIEEEALKDRTAEVRAQVPIPVAAFLLNEKRNSITKIELRTR
ARIVILPNDHLETPHFEVQRLRDDSPEAHSGQSSYEIAAAAAEVEEVQPAAATRTLVRQE
AAVKTAPARANAPVPTEVAAPVAAPAALPEPSLFKGLVKSLVSLFATKEEPVAPVVVEKP
ATERPARNEERRNGRQQSRNRNGRRDEERKPREERAPREERAPREERAPREAREETPTVA
REERAPREERAPRTPRAPREDRKPRGEREERVRELREPLDAAPAVAAEAVSEERPARQPR
EERAPREERQPRAPREERQPRAEQAAAASEEEVLTGEEQPQEDGQEGAEGDRPRRRSRGQ
RRRSNRRERQRDANGNVIEGSEETGENAEAATSEPTGAELAAGLAVTAAVASSVISAPAE
AQAHEQAERATAAVEETVIAETPVAETPIVEAPAVEASVAEAPVVEAPAVEATTPIEAPV
VPEVEVVPEVEVAPVQEAQPEVEVAAVEEAPAIEPQPVTETVVEAPVVEAAPEVREVREE
QTAFQWTAEPAAPVEAPEPAPVVEDAPAPVAEVVVAEPAPVVEPAPVVEPAPVVEAPVVA
EVAAPVVEAAPVSALTENGRAPNDPREVRRRRKEAEAAAAAAAAQEAEHESKPLV