Protein Info for PFLU_RS22950 in Pseudomonas fluorescens SBW25

Annotation: L-arabinose ABC transporter ATP-binding protein AraG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 PF00005: ABC_tran" amino acids 22 to 170 (149 residues), 98.1 bits, see alignment E=7.3e-32 amino acids 272 to 425 (154 residues), 69.8 bits, see alignment E=4e-23

Best Hits

Swiss-Prot: 86% identical to ARAG_PSEPF: Arabinose import ATP-binding protein AraG (araG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K10539, L-arabinose transport system ATP-binding protein [EC: 3.6.3.17] (inferred from 100% identity to pfs:PFLU4683)

MetaCyc: 61% identical to arabinose ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.17

Use Curated BLAST to search for 3.6.3.17 or 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0L2 at UniProt or InterPro

Protein Sequence (499 amino acids)

>PFLU_RS22950 L-arabinose ABC transporter ATP-binding protein AraG (Pseudomonas fluorescens SBW25)
MTGAALRFNGIGKEFPGVKALAQISFEARPREVHALMGENGAGKSTLLKILGGAYIPSSG
TLQIGEQTMAFKNAADSIASGVAVIHQELHLVPEMTVAENLFLGHLPSRFGVVNRSLLRK
QALACLKGLADEIDPEEKLGRLSLGQRQLVEIAKALSRGAHVIAFDEPTSSLSAREIDRL
MAIITRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRYVRTFDDMSALTHDQLVTCMVG
RDIQDIYDYRPRERGEVALKVDGLLGPGLREPVSLQVHKGEILGLFGLVGAGRTELFRLL
SGLTRSTAGSLELCGQKLPLHSPRDAIAAGVLLCPEDRKKEGIIPLSSVAENINISARGA
HSTFGWLLRDGWEKRNADQQIQAMKVKTPNAEQKIMYLSGGNQQKAILGRWLSMPMKVLL
LDEPTRGIDIGAKSEIYQIIHNLAASGIAVIVVSSDLMEVMGISDRIVVMSEGALTGELT
RDQADEARLLQLALPRSRA