Protein Info for PFLU_RS22650 in Pseudomonas fluorescens SBW25

Annotation: molybdopterin-synthase adenylyltransferase MoeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 PF14464: Prok-JAB" amino acids 8 to 119 (112 residues), 58 bits, see alignment E=1.2e-19 PF00899: ThiF" amino acids 252 to 487 (236 residues), 235.4 bits, see alignment E=6.8e-74 PF00581: Rhodanese" amino acids 526 to 611 (86 residues), 50.5 bits, see alignment E=3.6e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4624)

Predicted SEED Role

"Sulfur carrier protein adenylyltransferase ThiF" in subsystem Thiamin biosynthesis

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K120 at UniProt or InterPro

Protein Sequence (620 amino acids)

>PFLU_RS22650 molybdopterin-synthase adenylyltransferase MoeB (Pseudomonas fluorescens SBW25)
MDVLRPTALEQIYAHARRSYPEECCGFIFADGSVYLGSNIQNELHRKNPEMYPRSAANGY
TFSVADTLLMNKAFRSDNPVVVIYHSHPDVGAYFSDEDQDKALFLGEPIYPVSYLVVDVR
QGQALGSKLFAWDGKHFALQPFNDLHTELSMNAVSFPDILVRVAKLPESTLEGTGSTLRE
VIENLCCSHPQLRSHLFHEKNNQLKEHFLFTAEEALIDADEPLPQKAKIEVLLATSGGMD
VDALSNEEVQRYVRHITLPGVGREGQLNLKKAKVLIIGTGGLGSPISLYLAAAGIGTLGL
VDFDVVESSNLQRQIVHGNSTLGMPKVESAKQRLQDLNGHIQINAHDTALNADNALELVG
AYDLVIDGTDNFDTRYLVNDACVQLGKPLVYGAIYRFDGQISVLNYQGGPCYRCLFPSPP
PAELAPNCSAGGVIGVLPGVVGMIQATEAIKLLIGIGEPLSGRLMRFDALAMKFSEIRFK
RRADCPCCSEARHSETLAPAVCADAVLNPPSLAEERYIKPRVLKQVLEHPSSADVLLDVR
DASELEVCKLPGVVHIPLAELDGHLDSLSRDNTHYLICYAGSRAEQAASTLLAAGFANTK
VLQGGMKHWVRDVEPDMPLY