Protein Info for PFLU_RS22540 in Pseudomonas fluorescens SBW25

Annotation: GGDEF domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 315 to 337 (23 residues), see Phobius details PF03924: CHASE" amino acids 87 to 272 (186 residues), 122.6 bits, see alignment E=5.3e-39 PF00989: PAS" amino acids 367 to 492 (126 residues), 38.5 bits, see alignment E=3.1e-13 TIGR00229: PAS domain S-box protein" amino acids 376 to 503 (128 residues), 30.9 bits, see alignment E=2.6e-11 PF13426: PAS_9" amino acids 379 to 495 (117 residues), 24.6 bits, see alignment E=7.6e-09 PF08447: PAS_3" amino acids 534 to 607 (74 residues), 35.5 bits, see alignment E=2.9e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 635 to 793 (159 residues), 149 bits, see alignment E=1e-47 PF00990: GGDEF" amino acids 639 to 791 (153 residues), 140 bits, see alignment E=1.8e-44

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4600)

Predicted SEED Role

"CHASE domain/PAS domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZH6 at UniProt or InterPro

Protein Sequence (798 amino acids)

>PFLU_RS22540 GGDEF domain-containing protein (Pseudomonas fluorescens SBW25)
MPLQAVRPKILGFISEQASAWLVALVVLLAGFTLTAIVAWAASDLYQQQVRQRFQLLVNE
RYTRLQERFEDQEQRLNSLRRFFVNSDEVSREEFDGFALPLLLRARAYSWAPRVYREQRE
QFEQTVSDQRGTPFVIRELNSAGELAPAPERDEYVPVLYSQTQSLLGAPLGFDLLAQPVR
RSALERAQKTGKLAVSQPMQLVGVEPAYSTGVLLVAPVSRLPTSEQSQNEPYGYVMAVIS
MRQLVADGLPKPDRDNLTMQIVDTSDLQKRVLFESGTVVSDSDLVGARRLTLGDHVYALS
LRPSQVFDQANHSSLSTVLTMGGLLSLLLSALLYMLVSQRQRALKLVEQRTVQLRLREQE
LRGAHGQLRSVLNAATQVAIIATDLRGIINTFNAGAEQMLGFQAEHVVGTYTLESLHLAT
ELEVRAASLSVALGKRIPASQAMLVESPDNLHEAREWTLIRQDGSPLTVNMLATVLLDDH
GLWIGHLAIYLDITEQKRAYEALAARDRLLKKLSAHVPGGIFQFTLEPQDNWKFIYASDG
MRDIYEIEPGVLQQDAKKVFERIHPLDAERVRASIRLSALQLSHWREEYRVQLPQRGLRW
IRGEATPEELPAGGTLWHGYVSDISDLKRVEEELRALSITDSLTGIHNRRYFQDRLKAEM
VRLNRTSGALSVIMLDIDHFKRINDQHGHAVGDGVLQELCKRISQRLRRTDVFCRLGGEE
FMVLCPHTDGDQAYSLATELWASLRSAPMEPVGIVTASFGVASWRVDEGIDGLLLRADSA
VYVAKQGGRDRVEAPPVC