Protein Info for PFLU_RS22155 in Pseudomonas fluorescens SBW25

Annotation: NCS1 family nucleobase:cation symporter-1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 28 to 53 (26 residues), see Phobius details amino acids 60 to 79 (20 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 114 to 136 (23 residues), see Phobius details amino acids 152 to 172 (21 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 220 to 241 (22 residues), see Phobius details amino acids 261 to 285 (25 residues), see Phobius details amino acids 305 to 329 (25 residues), see Phobius details amino acids 343 to 363 (21 residues), see Phobius details amino acids 369 to 393 (25 residues), see Phobius details amino acids 419 to 442 (24 residues), see Phobius details amino acids 449 to 466 (18 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 24 to 454 (431 residues), 277.7 bits, see alignment E=8.4e-87

Best Hits

KEGG orthology group: K03457, nucleobase:cation symporter-1, NCS1 family (inferred from 100% identity to pfs:PFLU4518)

Predicted SEED Role

"Cytosine/purine/uracil/thiamine/allantoin permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K131 at UniProt or InterPro

Protein Sequence (480 amino acids)

>PFLU_RS22155 NCS1 family nucleobase:cation symporter-1 (Pseudomonas fluorescens SBW25)
MTEQLPKGYSPRLYNQDLGPLPQKWNWYNIFAFWMSDVHSVGGYVFAASLFALGLASWQV
LIALLAGICIVQLIANLVAKPSQQAAVPYPVICRLAFGVFGANIPAVIRGLIAVAWYGIQ
TYLASSALIIVVLRFFPQMAVYAEPHFAGLSYLGWFGFLSLWLLQAAVFWAGMESIRRFI
DWAGPVVYAVMFALAGWIVWKAGWSNISFTLAEKSLSGWQAFGQVIVATALVVSYFSGPT
LNFGDFSRYCRSMNDVRRGNFWGLPVNFLAFSLVTVVIVSGTLPVFGEMLHDPIATVSRI
DNNVAVLLGAFAFVTATIGINIVANFVSPAFDFANVAPSKISWRAGGMIAAVASIFITPW
NLFNNPLMIHYTLDILAAFIGPLFGILLVDFYLIKKQKIDVDALFDDSPSGRYYFDGGVN
WTAVKALVPATLAGVAITFTPALQGMANFAWFTGCFLGGLFFLVLARREQVRAPAPLVAG