Protein Info for PFLU_RS21920 in Pseudomonas fluorescens SBW25

Annotation: DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 6 to 595 (590 residues), 823.1 bits, see alignment E=1.3e-251 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 8 to 456 (449 residues), 508.3 bits, see alignment E=2.1e-156 PF00270: DEAD" amino acids 22 to 182 (161 residues), 85 bits, see alignment E=1.3e-27 PF00271: Helicase_C" amino acids 218 to 323 (106 residues), 65.8 bits, see alignment E=9.9e-22 PF16124: RecQ_Zn_bind" amino acids 335 to 396 (62 residues), 73.3 bits, see alignment 5.5e-24 PF09382: RQC" amino acids 401 to 510 (110 residues), 127.5 bits, see alignment E=5.5e-41 PF00570: HRDC" amino acids 530 to 595 (66 residues), 78.1 bits, see alignment E=9.6e-26

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to pfs:PFLU4468)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0X4 at UniProt or InterPro

Protein Sequence (708 amino acids)

>PFLU_RS21920 DNA helicase RecQ (Pseudomonas fluorescens SBW25)
MLEQAQRVLKDIFGYDSFRGRQGAIIERVASGGDALVLMPTGGGKSLCFQVPALLRNGLA
VVVSPLIALMDDQVATLEELGVAAASLNSTLSAEQQRDLAARIKRGEVKMLYLAPERLVQ
PRMLAFLQSLEIALFAIDEAHCVSQWGHDFRREYLQLGQLAELFPDVPRIALTATADKRT
REEIVERLHLQNAERFLSSFDRPNIFYRIVPKEQPRKQLLAFLSERRSDAGIVYCLSRKK
VDEVAAFLCEQGYPALPYHAGLPNETRSAHQKRFLNEEGLIMVATIAFGMGIDKSNVRFV
AHMDLPKSLEAYYQETGRAGRDGLPADAWMVYGLQDVVMLKQMLQNSEGDERHKRLEQHK
LDAMLSLCEETRCRRQTLLAYFDEDMPQPCGHCDNCVDGVQTWDATEPARQGLSAIYRTG
QRYGVGHLVDVLLGKDNEKVRSFGHEKLSVYGVGKARAEGEWRSLFRQMVARGLVDIDIE
GYGGLRLNDSCRPLLKGEVSLELRRDLKPQTTAKSSTSQASQLVRGEEREQWEALRTLRR
KLAQEHSVPPYVIFPDSTLLEMLREQPTSMAEMARVSGVGARKLERYGQAFLEVLGGQAE
APKEIADIRHELISLARAGMTPIQIAGQLQCSEKNVYTLLAESIGKQQLSLEQALDLPED
LLGEIQDAFLDGEGELPPVAEIAPLFTGRVPEGVLYCVRAALQSEFEI