Protein Info for PFLU_RS21775 in Pseudomonas fluorescens SBW25

Annotation: flagellar basal body M-ring protein FliF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 transmembrane" amino acids 37 to 57 (21 residues), see Phobius details amino acids 488 to 510 (23 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 29 to 592 (564 residues), 312.5 bits, see alignment E=3.6e-97 PF01514: YscJ_FliF" amino acids 58 to 234 (177 residues), 219.3 bits, see alignment E=3.5e-69 PF08345: YscJ_FliF_C" amino acids 267 to 466 (200 residues), 148.4 bits, see alignment E=2.1e-47

Best Hits

Swiss-Prot: 76% identical to FLIF_PSEAE: Flagellar M-ring protein (fliF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 100% identity to pfs:PFLU4439)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0U5 at UniProt or InterPro

Protein Sequence (593 amino acids)

>PFLU_RS21775 flagellar basal body M-ring protein FliF (Pseudomonas fluorescens SBW25)
MAEAVVDNAPAKADGKPPLFGLSFLENLSEMTMLRQVGLMVGLAASVAIGFAVVLWSQQP
DYRPLYGSLAGMDSKQIMETLAAADIAYTVEPNSGALLVKADDVARARMKLAAAGVTPSD
SNIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTISSLNNVKGARVHLAIPKSSVFVR
DERKPSASVLVELFSGRSLEPGQVLAIVNLVATSVPELSKSQITVVDQKGNLLSDQGENS
ALTQAGKQFDYSRRVESMLTQRVHNILQPVLGNDRYKAEVSADVDFSAVESTSEQFNPDQ
PALRSEQSTSEQRTAANGPPQGVPGALSNQPPAPASAPQTTGGAAATAGAIQPGQPLLDA
NGQQIMDPATGQPMLAPYPADKRNQSTKNFELDRSISHTKQQQGRVNRLSVSVVVDDQVK
VNAADGAVTRAPWSADELARFTRLVQDAVGFDASRGDSVSVINMPFSAERGEVIAEPAFY
SQPWFWDIVKQVLGVLFILVLVFGVLRPVLNNITGNGKKQLAAFGGSDVELGGMGGLDGE
LANDRVSLGGPQSILLPSPSEGYDAQLNAIKSLVAEDPGRVAQVVKEWINADE