Protein Info for PFLU_RS21645 in Pseudomonas fluorescens SBW25

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 transmembrane" amino acids 598 to 615 (18 residues), see Phobius details PF01627: Hpt" amino acids 8 to 99 (92 residues), 64 bits, see alignment E=2.4e-21 PF02895: H-kinase_dim" amino acids 352 to 411 (60 residues), 57.3 bits, see alignment 3.4e-19 PF02518: HATPase_c" amino acids 459 to 598 (140 residues), 66.2 bits, see alignment E=6.8e-22 PF01584: CheW" amino acids 605 to 732 (128 residues), 96.6 bits, see alignment E=2e-31

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 95% identity to pfs:PFLU4414)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0H7 at UniProt or InterPro

Protein Sequence (738 amino acids)

>PFLU_RS21645 chemotaxis protein CheA (Pseudomonas fluorescens SBW25)
MSFGADEEILQDFLVEAGEILEQLSEQLVELESRPDDANLLNAIFRGFHTVKGGAGFLQL
HELVECCHIAENVFDILRKGERHVDSELMDVILEALDAVNGMFSEVRERAPITAATPELL
AALARYAEPADLSAAPVAEAAPEPVAEPEADVTDSEFEQLLNSLSAVKAEAEAPAAVAPA
AAPTSEDITDAEFESLLDQLHGKGQFAADAVPPAAAAAAPAAPVSTDITDDEFEALLDQL
HGKGTFAADALPAVAATPAAAASTAAPAAAAPAGDGLISDHEFESLLDELHGKGKFSEVA
PAAAVATAAPVAAKAAAPAPAAKPAPAPAAAPAPARAAAAPVAEKPASEAETTVRVDTAR
LDDIMNMVGELVLVRNRLVRLGLSSGDEAMQKAVSNLDVVTADLQTAVMKTRMQPIKKVF
GRFPRLVRDLARQLKKEINLELVGEETDLDKNLVEALADPLVHLVRNAVDHGVETPEERE
ASGKSRGGKVILAAEQEGDHILLSITDDGKGMDPNILRSIAVKRGVMDKDAADRLTDTEC
YNLIFAPGFSTKTEISDVSGRGVGMDVVKTKISQLNGSINIYSTKGQGSKIVIKVPLTLA
IMPTLMVMLGNQAFAFPLVNVNEIFHLDLSRTNVVDGQEVVIVRDKALPLFYLKRWLVAS
AAHEEQREGHVVILSVGTQRIGFVVDQLVGQEEVVIKPLGKMLQGTPGMSGATITGDGRI
ALILDVPSMLKRYAARRI