Protein Info for PFLU_RS21460 in Pseudomonas fluorescens SBW25

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01891: amidohydrolase" amino acids 49 to 430 (382 residues), 321.1 bits, see alignment E=5.1e-100 PF01546: Peptidase_M20" amino acids 106 to 439 (334 residues), 129.8 bits, see alignment E=1.4e-41 PF07687: M20_dimer" amino acids 239 to 335 (97 residues), 40.8 bits, see alignment E=1.9e-14

Best Hits

KEGG orthology group: K01436, amidohydrolase [EC: 3.5.1.-] (inferred from 100% identity to pfs:PFLU4376)

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-

Use Curated BLAST to search for 3.5.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0E0 at UniProt or InterPro

Protein Sequence (445 amino acids)

>PFLU_RS21460 amidohydrolase (Pseudomonas fluorescens SBW25)
MRLTGLTHTWVFGALCAVASSAVIAASSGQDSAREEIAAQAKALEPALLETRRDLHAHPE
LGNTETRTAELVAKQLRDLGLEVKTGVARTGVVAVLKGALPGPTVALRADMDALPVKEVA
DLPFASNAKGTYLGKEVDVMHACGHDAHVAILLSTAKILTGMRERLPGTVVFYFQPAEEG
PSDFIPDGKNTWGAKMMVQEGVMKAPKPDAVFGLHVWAGVPAGQIAYRPGPTLASSDDLR
IKILGKQTHAGRPWDGIDPITVGAQTIVGLQTVVSRRTDISSFPSVVSIGTINGGTRYNI
IPESVDMTGTIRSYDYGIRQKLHADVRQTVEKIAESGGAKAEVTIIEKYDPTINNPALTE
KMLPSLRWAAKDDVVQGPLVGGAEDFSFYAKEAPGLFVFLGVTPRDQDMSKAAPNHNPGF
FVDESALVVGVRTLASLATDYLYTQ