Protein Info for PFLU_RS21015 in Pseudomonas fluorescens SBW25

Annotation: alpha/beta fold hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 346 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 80 to 177 (98 residues), 29.3 bits, see alignment E=1.7e-10 PF12146: Hydrolase_4" amino acids 81 to 175 (95 residues), 32.6 bits, see alignment E=1.3e-11 PF12697: Abhydrolase_6" amino acids 82 to 293 (212 residues), 36.7 bits, see alignment E=1.8e-12 PF00326: Peptidase_S9" amino acids 97 to 185 (89 residues), 31.8 bits, see alignment E=2.7e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4278)

Predicted SEED Role

"Probable lipoprotein signal peptide"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K032 at UniProt or InterPro

Protein Sequence (346 amino acids)

>PFLU_RS21015 alpha/beta fold hydrolase (Pseudomonas fluorescens SBW25)
MKTAFRALFLLCLATPAFADERSVGFTTTTLPAAQTNRALEMVVWYPAATTVQPQLIADN
AVFIGALAVPDAPPASGDHPLVVLSHGYGGNWGKQVWLASALAHKGYIVAAINHPGTTTQ
DRSPQNAAQLWQRPADLSRAIDAVLAQPKQFGAVAKNQIAAVGHSLGGWTVLEIAGARFD
PDLFSRDCAVHPKLGGCIGYREMNPAGTPDGKAQLAADLRDKRVTAIVSLDLGLSRGMTD
ASLAALQVPTLVIAGGVPTEDMDFNLESADLAKRLPEASTQYVKIDDATHFSFMPICKPG
GMALVEESSPGDGMICRDGEGARPRAVIQQQVIALISEFLQRTAPP