Protein Info for PFLU_RS20695 in Pseudomonas fluorescens SBW25

Annotation: M4 family metallopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF01447: Peptidase_M4" amino acids 81 to 179 (99 residues), 106.9 bits, see alignment E=1.3e-34 PF02868: Peptidase_M4_C" amino acids 182 to 351 (170 residues), 187.4 bits, see alignment E=1.8e-59

Best Hits

Swiss-Prot: 61% identical to PRT1_PECCC: Extracellular metalloprotease (prt1) from Pectobacterium carotovorum subsp. carotovorum

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4216)

Predicted SEED Role

"putative metalloprotease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZV8 at UniProt or InterPro

Protein Sequence (353 amino acids)

>PFLU_RS20695 M4 family metallopeptidase (Pseudomonas fluorescens SBW25)
MCVRHPRHPIFCLIPPYMLDQIARNGDKAQRDVALRTRAKDSTFRSLRMVAVPAKGPARM
ALVMGADKRRSIYSAEGTDSLPGKLVRGEGQPASGDAAVDEAYDGLGATFDFFDQVFDRN
SIDDAGMALDATVHFGQNYNNAFWNSTQMVFGDGDEQLFNRFTVALDVIGHELAHGVTED
EAKLMYFNQSGALNESLSDVFGSLIKQYALKQTAEDADWLIGKGLFTKKIKGTALRSMKA
PGTAFDDKLLGKDPQPGHMDDFVQTYEDNGGVHINSGIPNHAFYQVATKLGGFAWERAGR
IWYDALRDARLRPNSGFLRFARITYDVAGRLYGANKDEQKAVKDGWKAVGISV