Protein Info for PFLU_RS20570 in Pseudomonas fluorescens SBW25

Annotation: class I SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF05175: MTS" amino acids 35 to 146 (112 residues), 24.2 bits, see alignment E=9.6e-09 PF03141: Methyltransf_29" amino acids 40 to 144 (105 residues), 24.9 bits, see alignment E=3e-09 PF13489: Methyltransf_23" amino acids 43 to 192 (150 residues), 67.2 bits, see alignment E=5.9e-22 PF01209: Ubie_methyltran" amino acids 43 to 157 (115 residues), 72 bits, see alignment E=2e-23 PF13847: Methyltransf_31" amino acids 45 to 149 (105 residues), 75.9 bits, see alignment E=1.2e-24 PF08241: Methyltransf_11" amino acids 49 to 145 (97 residues), 97.1 bits, see alignment E=3.1e-31 PF13649: Methyltransf_25" amino acids 49 to 141 (93 residues), 86.3 bits, see alignment E=7.4e-28 PF08242: Methyltransf_12" amino acids 49 to 143 (95 residues), 63.3 bits, see alignment E=1.1e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4194)

Predicted SEED Role

"SAM-dependent methyltransferase YafE (UbiE paralog)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZT8 at UniProt or InterPro

Protein Sequence (254 amino acids)

>PFLU_RS20570 class I SAM-dependent methyltransferase (Pseudomonas fluorescens SBW25)
MTATVHTQVVQKQFGEQASAYLSSAVHAQGTEFALLQAELVGQGAARLLDLGCGAGHVSF
NMAPLVKEVVAYDLSQQMLDVVATAAVDRGFENIRTVQGAAERLPFADGEFDFVFSRYSA
HHWSDLRLALREVRRVLKPGGVVAFVDVLSPGSPLLDTYLQTAEVLRDTSHVRDYSAAEW
MQQLSETGLHVRNSSRQRLRLEYTSWVERMRTPEVLRAAILELQQAMGQEVRDYYEIQAD
GTFSTDVLVVFAER