Protein Info for PFLU_RS20380 in Pseudomonas fluorescens SBW25

Annotation: sugar ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00532: Peripla_BP_1" amino acids 36 to 296 (261 residues), 86.2 bits, see alignment E=4.3e-28 PF13407: Peripla_BP_4" amino acids 38 to 301 (264 residues), 206.8 bits, see alignment E=6.8e-65 PF13377: Peripla_BP_3" amino acids 171 to 306 (136 residues), 38.4 bits, see alignment E=2.1e-13

Best Hits

KEGG orthology group: K10439, ribose transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU4160)

Predicted SEED Role

"Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1)" in subsystem D-ribose utilization (TC 3.A.1.2.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZP9 at UniProt or InterPro

Protein Sequence (319 amino acids)

>PFLU_RS20380 sugar ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25)
MKLPFAGRLLAVAVLAAASAALPLSSAFADDAAAKPKVGLVMKSLANEFFVTMQDGAKAY
QKDHAADFDMITNGIKNETDTSAQIDIVNQMILSKVNAIVIAPADSKALVTVLKKASDAG
IKVVNIDNRLDPDVLKSKNLNIPFVGPDNRKGSRLVGDYLAKQLASGDKVGIIEGVPTTT
NAQQRTAGFKDAMDAAGMKIVSTQSGNWEIDQGQKVASAMLSEYPDLKALLAGNDNMALG
AVSAVRAAGKAGKVLVVGYDNIEAIKPMLQDGRVLATADQAAAQQAVFGIQNALKLVKGE
KVDSKDGVIETPVELVLKK