Protein Info for PFLU_RS20100 in Pseudomonas fluorescens SBW25
Annotation: aldehyde dehydrogenase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to PCHA_PSEPU: 4-hydroxybenzaldehyde dehydrogenase (NADP(+)) (pchA) from Pseudomonas putida
KEGG orthology group: K00128, aldehyde dehydrogenase (NAD+) [EC: 1.2.1.3] (inferred from 100% identity to pfs:PFLU4112)MetaCyc: 60% identical to 4-hydroxybenzaldehyde dehydrogenase (Pseudomonas putida NCIMB 9866)
HYDROXYBENZALDEHYDE-OXIDATION-NADP-RXN [EC: 1.2.1.96]; 1.2.1.96 [EC: 1.2.1.96]
Predicted SEED Role
"Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28)" in subsystem Phenylpropanoid compound degradation (EC 1.2.1.28)
MetaCyc Pathways
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- octane oxidation (4/5 steps found)
- phytol degradation (3/4 steps found)
- putrescine degradation III (3/4 steps found)
- toluene degradation III (aerobic) (via p-cresol) (8/11 steps found)
- ethanol degradation III (2/3 steps found)
- hypotaurine degradation (2/3 steps found)
- 4-methylphenol degradation to protocatechuate (2/4 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (2/4 steps found)
- fatty acid α-oxidation I (plants) (2/4 steps found)
- serotonin degradation (4/7 steps found)
- 1,3-dimethylbenzene degradation to 3-methylbenzoate (1/3 steps found)
- 1,4-dimethylbenzene degradation to 4-methylbenzoate (1/3 steps found)
- 3-chlorotoluene degradation II (1/3 steps found)
- D-phenylglycine degradation (1/3 steps found)
- histamine degradation (1/3 steps found)
- toluene degradation to benzoate (1/3 steps found)
- 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
- noradrenaline and adrenaline degradation (8/13 steps found)
- benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
- dopamine degradation (2/5 steps found)
- aromatic biogenic amine degradation (bacteria) (4/8 steps found)
- benzoate biosynthesis II (CoA-independent, non-β-oxidative) (1/4 steps found)
- 4-hydroxymandelate degradation (2/6 steps found)
- mandelate degradation I (1/5 steps found)
- ceramide degradation by α-oxidation (2/7 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- alkane oxidation (1/6 steps found)
- salicin biosynthesis (1/6 steps found)
- 2,4-xylenol degradation to protocatechuate (1/7 steps found)
- limonene degradation IV (anaerobic) (1/7 steps found)
- mandelate degradation to acetyl-CoA (7/18 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
- toluene degradation IV (aerobic) (via catechol) (2/13 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
- superpathway of aerobic toluene degradation (10/30 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (13/42 steps found)
KEGG Metabolic Maps
- 1,2-Dichloroethane degradation
- 3-Chloroacrylic acid degradation
- Arginine and proline metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via hydroxylation
- Biosynthesis of phenylpropanoids
- Butanoate metabolism
- Fatty acid metabolism
- Glycerolipid metabolism
- Glycolysis / Gluconeogenesis
- Histidine metabolism
- Limonene and pinene degradation
- Lysine degradation
- Propanoate metabolism
- Pyruvate metabolism
- Toluene and xylene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.28, 1.2.1.3
Use Curated BLAST to search for 1.2.1.28 or 1.2.1.3 or 1.2.1.96
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3JZK0 at UniProt or InterPro
Protein Sequence (492 amino acids)
>PFLU_RS20100 aldehyde dehydrogenase family protein (Pseudomonas fluorescens SBW25) MTIPTFATYAISGDQYINGTWRTGRSARRLDDRNPFNGECLLEMPLASVADLEDAYQAAQ RAQIDWAALHPTQRGAQLEKLAQVIQDRSEEIIDWLIRESGSTRIKAGMEWQFTLNLVRE CATLPMQVEGRILSSYKPGEQSFVFREPLGVVGVISPWNFPLYLSMRSVVPALALGNTVV LKPASDTAVTGGLLIAHLFEEAGFPAGSLNVVVGAGSEIGDAFVEHPIPSLISFTGSTDV GRNVGRIATGGKHIKRVALELGGNAPLVVLDDADIEIAAHAAVVGRFLHQGQICMSVNRV IVDRSLYADFAALVVERVRNLKTGDPAKADTVIGPVVNQSQLDGLLGKIDAAERAGLKQL CGGQAQGLVLPAHVLGDVGPDQALARDETFGPLLPLMIAHNQAHALELANASEYGLSSAV FTRDMARGLGFARGIVAGMTHINDITVDDQPNAPFGGEKNSGLGRFNGHYALDEFTRAHW VTWQAGSHRYPF