Protein Info for PFLU_RS19950 in Pseudomonas fluorescens SBW25

Annotation: beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 916 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 71 to 314 (244 residues), 105.1 bits, see alignment E=1e-33 PF01915: Glyco_hydro_3_C" amino acids 349 to 735 (387 residues), 176.1 bits, see alignment E=1.8e-55 PF07691: PA14" amino acids 435 to 583 (149 residues), 93.3 bits, see alignment E=2.6e-30 PF14310: Fn3-like" amino acids 772 to 842 (71 residues), 83.5 bits, see alignment 1.8e-27

Best Hits

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to pfs:PFLU4082)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZF0 at UniProt or InterPro

Protein Sequence (916 amino acids)

>PFLU_RS19950 beta-glucosidase (Pseudomonas fluorescens SBW25)
MNKRKMIGAQSAFALLALAVSQVHAATSPALDEGRVSRAEKAADKTLAKMTMEEKLAYIG
GTGGWDVKPLTQYGIPQIHGADGGVGVRYTSEGNAQGVVYPSGPNLAASWNPRRAIDLGR
ALGYDTASGGYQFVTGPGVNLYRMPYSGRAFEYLSGEDPFLGASLGPAVINGIQSRGVWA
NAKHFAANDQESNRFHLDEIISERVLREMTLPPFESASKNSKVAMMMCAFQKVNGEFACQ
NKHLMRDILKTEWGYPGFVQSDYNAVVNGLPAAQAGTDLDMMGYQMNSTILKPYLDSGEL
SSATIDDKVRRILKQIYLYKFDSKAPLTSHNMNSATSNRVALNAAREGIVLLKNQNSLLP
LDAKKVKRIAVVGTLAKYAPPTGFGSANVMAANYISELSGLQQLAPGAKVEFIDGLSLDP
ATSTWTHADSNGNSAKGLKTEFFSNTNWSGDPAASRTDTHVDLDWSTDTLPVNGDTAATS
IRYSGQITPTVSGEQVFKIRADGAVRLYVNGKKVLDNGDGKPLPNNSIPPTIPVFAKVNL
EAGKPVDIKLEYSRRNGYLSTMGGLVGVQMSWASLVAPQDLAQYDAVLVAAGNSNEYEGE
GFDHSFDLPEYQNLLIQSIAKVNPNTVVTLHGGTGLKMSDWIDQVPAALHAFYPGQNGGQ
ALAEILLGKVNPSAKLPISIERNIEDNPIYATFPKFDNQETLAEMSYKDDLFLGYRGYEK
KGIKPLYPFGYGLSYTTFGYSNISVTPGVAVAGAPIKVSFDLSNTGKVAGAEVAELYVGQ
NNPKVERAIKELKGYKKVFLKPGESKRVTIELNDRSLAYYDVASKQWVVDADSFNLSVGA
SSQDIRLNAKLVNPFRQELSTTTSNPLPRSALNSTLRESTVKTGGVLHQNEGDSGTSDGD
GYDMSDDSSQGSAAGN