Protein Info for PFLU_RS19930 in Pseudomonas fluorescens SBW25

Annotation: type II secretion system protein GspG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 141 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details PF07963: N_methyl" amino acids 10 to 34 (25 residues), 31.7 bits, see alignment 8e-12 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 12 to 35 (24 residues), 28 bits, see alignment 1.4e-10 TIGR01710: type II secretion system protein G" amino acids 14 to 141 (128 residues), 96.6 bits, see alignment E=1.2e-31 PF08334: T2SSG" amino acids 38 to 140 (103 residues), 101.4 bits, see alignment E=2.9e-33

Best Hits

Swiss-Prot: 49% identical to GSPG_XANCP: Type II secretion system protein G (xpsG) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02456, general secretion pathway protein G (inferred from 100% identity to pfs:PFLU4078)

Predicted SEED Role

"General secretion pathway protein G"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZE6 at UniProt or InterPro

Protein Sequence (141 amino acids)

>PFLU_RS19930 type II secretion system protein GspG (Pseudomonas fluorescens SBW25)
MRPTRFKPARRQGGFTLLEMLAVIVLLGIVATIVVRQVGGNVDKGKYGAGKAQLASLSMK
IESYGLDVGSPPKTLQQLVEKPGNSAGWAGPYAKPSDLKDPFGHAFGYRFPGEHGAFDLI
FYGQDGQPGGEGYSADLGNWE