Protein Info for PFLU_RS19740 in Pseudomonas fluorescens SBW25
Annotation: NAD-dependent succinate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to GABD_ECOLI: Succinate-semialdehyde dehydrogenase [NADP(+)] GabD (gabD) from Escherichia coli (strain K12)
KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 100% identity to pfs:PFLU4039)MetaCyc: 59% identical to succinate-semialdehyde dehydrogenase (NADP+) GabD (Escherichia coli K-12 substr. MG1655)
SUCCSEMIALDDEHYDROG-RXN [EC: 1.2.1.79]; Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.79, 1.2.1.20]
Predicted SEED Role
No annotation
MetaCyc Pathways
- L-lysine degradation IV (5/5 steps found)
- superpathway of 4-aminobutanoate degradation (3/3 steps found)
- L-lysine degradation X (5/6 steps found)
- 4-aminobutanoate degradation II (2/2 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
- 4-hydroxyphenylacetate degradation (6/8 steps found)
- superpathway of L-arginine and L-ornithine degradation (9/13 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (7/11 steps found)
- L-lysine degradation I (4/7 steps found)
- nicotine degradation II (pyrrolidine pathway) (6/11 steps found)
- L-lysine degradation III (2/6 steps found)
- nicotine degradation I (pyridine pathway) (5/17 steps found)
- superpathway of L-lysine degradation (17/43 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.20
Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20 or 1.2.1.79
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3JZA7 at UniProt or InterPro
Protein Sequence (486 amino acids)
>PFLU_RS19740 NAD-dependent succinate-semialdehyde dehydrogenase (Pseudomonas fluorescens SBW25) MLKNRLQDPSLLAELAYVDGQWIAADSAATLDIRDPATGHVLAQVPAMDAVDTRRAIEAA ERAWPAWRARPAAERAALLERWYQAMIDNLDDLALIMTCEQGKPLNEARGEVRYGAGFVK WFAEEARRVYGETMPAPSGDRRLLTLKQPVGVCAAITPWNFPNAMITRKCAPALAAGCPI IVKPSDLTPLSALALAVLAERVGIPAGVFNVITGLPAGIGEELTGNPAVRKISFTGSTAV GRLLMRQSAEHIKRLSLELGGNAPFIVFDDADLEQAIAGVMLSKFRNAGQTCVCANRILV QDGIYARFAARLVEEVGKLKVGNGLEDGVTIGPLINPAAVNKVARHIDDALSQGAQLLCG AIPSGDSQFVQPTVLGDTHVGMLLANEETFGPVAPLMRFTDEAEALALANATPYGLGAYY FTQDLQRSWRFGEALEFGMVGLNTGIISMEVAPFGGIKQSGLGREGSKYGLDEYLEVKAF HIGGLN