Protein Info for PFLU_RS19425 in Pseudomonas fluorescens SBW25

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 381 (345 residues), 158.7 bits, see alignment E=9e-51 PF16576: HlyD_D23" amino acids 47 to 264 (218 residues), 61.4 bits, see alignment E=1.6e-20 PF13533: Biotin_lipoyl_2" amino acids 64 to 115 (52 residues), 30.2 bits, see alignment 6.1e-11 PF13437: HlyD_3" amino acids 186 to 266 (81 residues), 40.3 bits, see alignment E=9e-14

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 100% identity to pfs:PFLU3977)

Predicted SEED Role

"pyoverdine-specific efflux macA-like protein" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYX2 at UniProt or InterPro

Protein Sequence (389 amino acids)

>PFLU_RS19425 efflux RND transporter periplasmic adaptor subunit (Pseudomonas fluorescens SBW25)
MKRPRPARRALLAIACLIPIIAVAAWQVLPPGRDALTTVAVTRGNIENSVTALGTLQPRR
YVDVGAQASGQIRKIHVEAGDHVQEGQLLVEIDPSTQKAKLDASRYAIENLKAQLQEQKA
QHELARQKYQRQQRLAAGNATREEDVQTAKAELSATQARVDMFQAQILQAQASLRSDEAE
LGYTRIYAPMTGTVVAVDARVGQTLNAQQQTPLILRIAKLSPMTVWAEVSEADIGHVKPG
MTAYFTTLSGGNRRWSSTVRQILPIPPKPLNETQGSGSPNSSSKSGSGRVVLYTVLLDVD
NSDNALMAEMTTQVFFVAGQVKDALTAPVAALQGTSSADRQIARVVTDNGRIEERHVRLG
ISDRLRVQVLDGLNEGDRLLIGPADGNGG