Protein Info for PFLU_RS19415 in Pseudomonas fluorescens SBW25

Annotation: SidA/IucD/PvdA family monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 PF13434: Lys_Orn_oxgnase" amino acids 10 to 356 (347 residues), 368.8 bits, see alignment E=4.1e-114 PF13454: NAD_binding_9" amino acids 16 to 168 (153 residues), 26.3 bits, see alignment E=9.8e-10

Best Hits

Swiss-Prot: 78% identical to PVDA_PSEAE: L-ornithine N(5)-monooxygenase (pvdA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10531, L-ornithine N5-oxygenase [EC: 1.13.12.-] (inferred from 100% identity to pfs:PFLU3975)

MetaCyc: 78% identical to L-ornithine 5-monooxygenase (Pseudomonas aeruginosa PAO1)
RXN-11128 [EC: 1.14.13.195]

Predicted SEED Role

"L-ornithine 5-monooxygenase (EC 1.13.12.-), PvdA of pyoverdin biosynthesis" in subsystem Siderophore Pyoverdine (EC 1.13.12.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.13.12.-

Use Curated BLAST to search for 1.13.12.- or 1.14.13.195

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYX0 at UniProt or InterPro

Protein Sequence (445 amino acids)

>PFLU_RS19415 SidA/IucD/PvdA family monooxygenase (Pseudomonas fluorescens SBW25)
MTQAIASPAVHDLIGIGFGPSNLALAIALQEREKAQGKLDVLFLDKQADYRWHGNTLVTQ
SELQISFLKDLVTLRNPTSPYSFVNYLKAHDRLVDFINLGTFYPCRMEYNDYLRWVAGQF
QAQARYGEEVLAIEPILHQQQVEALRVISRDALGEQHVRTTRSVVVSAGGTPRIPEAFKA
LKGDSRVFHHSQYLERMASQPCVEGKAMRIAIIGGGQSAAEAFIDLNDSFPSVQVDMILR
GSALKPADDSPFVNEVFSPAFTDLVFQQEGAERERLVAEYQNTNYSVVDLDLIERIYGIF
YRQKVSGIARQAFRTMTVVEKATPGPLGIELVLRNNANGETSVNHYDAVILATGYERQTH
RALLAPLEGYLGDFDVARDYRIVTDERCNAGIYIQGFSQASHGLSDTLLSVLPIRADEIA
ASLYALDRGRGKGRSVQDLLLATAS