Protein Info for PFLU_RS18955 in Pseudomonas fluorescens SBW25

Annotation: hydroxymethylglutaryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 7 to 277 (271 residues), 177.3 bits, E=2.3e-56

Best Hits

Swiss-Prot: 80% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 100% identity to pfs:PFLU3887)

MetaCyc: 80% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• mevalonate degradation (1/2 steps found)
• ketogenesis (3/5 steps found)
• cis-geranyl-CoA degradation (4/9 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Geraniol degradation
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.4

Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4

Sequence Analysis Tools

See C3JYE9 at UniProt or InterPro

Protein Sequence (299 amino acids)

>PFLU_RS18955 hydroxymethylglutaryl-CoA lyase (Pseudomonas fluorescens SBW25)
MSLPSHVRLVEVGPRDGLQNEAQPISVADKVRLVDALSAAGLSYIEVGSFVSPKWVPQMA
GSAEVFAQIQRRPGVTYGALAPNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISES
LERFAPIMAAARQHGISVRGYVSCVLGCPYEGEIAPAQVAAVARELYAMGCYEVSLGDTI
GTGTAGATRRLFEVVGAQVPRDKLAGHFHDTYGQAIANIYASLLEGIAVFDSSIAGLGGC
PYAKGASGNVATEDVLYLLNGLGIETGIDLQALIGAGQQISNVLGRPTGSRVAKARNTG