Protein Info for PFLU_RS18155 in Pseudomonas fluorescens SBW25
Annotation: glycerol kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 51% identical to APLK_PECAS: Apulose kinase (aplK) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to pfs:PFLU3723)MetaCyc: 51% identical to apulose kinase (Pectobacterium atrosepticum SCRI1043)
RXN-20928 [EC: 2.7.1.233]
Predicted SEED Role
"Unknown pentose kinase TM0952"
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- glycerol degradation I (3/3 steps found)
- D-apiose degradation I (2/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.30
Use Curated BLAST to search for 2.7.1.233 or 2.7.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3JXU8 at UniProt or InterPro
Protein Sequence (491 amino acids)
>PFLU_RS18155 glycerol kinase (Pseudomonas fluorescens SBW25) MTNNTPLILAIDEGTSNAKAVLVNERGQVIARGSRPLSITHPQPGFSEQDPLLIWYSTLA AIEECLAQVDRPITALAISNQRESVVAWDRHTGEVLSPCISWQCRRSTALCAQLHEQGHE ALILDKTGLALDPMFSAGKFRWILDHLEDGHARAAAGDICLGTVDSWLLWKLSGGQVFAT DYSNAARTQLFNLHTCAWDPVLLDLFQIPLAALAPIQPSAGFFAETVAAGGLPAGIPVMS MIGDSNAALYGQGGFAPGLVKATLGTGSSLMTPMGGPIASTHGLSTTLAWHDGGQPTFGM EGNIVHTGAAVQWASRLVAGESLDVLTEQAAQLPDNGGVYFVPALSGLGAPHWQADARGL ICGLTEVTSGAHILRASLESIGYQIRDVFEAMQQDIGSPLKELWVDGGATRNRWLMQFLA NLLQRPVVRSLSPEVSALGAAHLAGRALGVWVSDEALGALERQRERFEPQDDCAMAGRYS AWKKTLARTLL