Protein Info for PFLU_RS17585 in Pseudomonas fluorescens SBW25

Annotation: type IV secretion protein Rhs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1597 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 262 to 285 (24 residues), see Phobius details amino acids 301 to 321 (21 residues), see Phobius details PF20148: DUF6531" amino acids 431 to 503 (73 residues), 69.6 bits, see alignment 4.9e-23 TIGR01643: YD repeat (two copies)" amino acids 618 to 659 (42 residues), 17.9 bits, see alignment (E = 3.5e-07) amino acids 788 to 816 (29 residues), 23.2 bits, see alignment (E = 7.4e-09) amino acids 829 to 870 (42 residues), 17.4 bits, see alignment (E = 4.9e-07) amino acids 871 to 912 (42 residues), 33.1 bits, see alignment (E = 5.6e-12) amino acids 998 to 1022 (25 residues), 17.2 bits, see alignment (E = 5.7e-07) amino acids 1024 to 1056 (33 residues), 18.5 bits, see alignment (E = 2.2e-07) amino acids 1247 to 1281 (35 residues), 27 bits, see alignment (E = 4.4e-10) PF05593: RHS_repeat" amino acids 788 to 820 (33 residues), 23.5 bits, see alignment (E = 1.2e-08) amino acids 871 to 907 (37 residues), 27 bits, see alignment (E = 9.3e-10) amino acids 978 to 1018 (41 residues), 22.4 bits, see alignment (E = 2.7e-08) amino acids 1004 to 1036 (33 residues), 30.7 bits, see alignment (E = 6.7e-11) amino acids 1247 to 1278 (32 residues), 23.4 bits, see alignment (E = 1.3e-08) PF03527: RHS" amino acids 1329 to 1363 (35 residues), 56.8 bits, see alignment (E = 3e-19) TIGR03696: RHS repeat-associated core domain" amino acids 1355 to 1431 (77 residues), 94.1 bits, see alignment 5.9e-31 PF02917: Pertussis_S1" amino acids 1470 to 1527 (58 residues), 34.1 bits, see alignment (E = 5.3e-12)

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3606)

Predicted SEED Role

"Rhs family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXH8 at UniProt or InterPro

Protein Sequence (1597 amino acids)

>PFLU_RS17585 type IV secretion protein Rhs (Pseudomonas fluorescens SBW25)
MIELTSQTIGFLVLAALDSKTRDVDSVISDFKRCLNHYDAWAESFFSFSALDIEQVFKVG
HDVALVAPINHSLFPSTTTATCQANGTLTLVHMFQSARFVPIGNTPVMLQRIDPKGGPLG
DPLHKTIGPSGILEITECDRNQQYRVSFYPNVSTAHFKALYASYQSVIEPLEGWLRGEWG
TTFEPLWKGYSEANVLKRYLSLHQGYARGFGEALYSLWDNIQQMLQWFLDPLGHAEKLLH
YLSQDEFEKLLKLGTESLAKGLMVLSDEPLLFVYLSAMVSWMRMLPPPYMNELLGEISAE
VLINVVLCLATAGMGMAVRIGTKVLGSIKSQRARRWLEHMAGQFGKFRADDHTQVAKPIL
LSSQATTIKTIPDVPLKAGDQVVANPVPVVRDKAKQRTVLVRQEPVDDVPASARNPNGDA
AAFSDKTATNGCPVSMVTGEELLTLTDGALDGVLPFEWTRLYRTSAVEVDCGLGFGWSHS
LAHRLAVSGNSVVWTDHENRSTTLPLPSVSRPAITNSLAEAAIYLGAVPDELVLAQSSRF
YHFRDGALTAISDAYDNRLRIARDFLGRIERLDNGVGRSLFLRYASGRIVAVDYQIQRIV
DEGPYVWVTEQTVVSYAYDGAGRLVSATNAVGESEVYRYDEQHVILERGLAGGASFYWEW
EQAGKAARCVRHWASFSQMDTRYAWDDNGQVTVFNADGSQEIYVHDQRARLVQRVDPDGA
EHFKSYDEKGRLTVEQDPLGAVKAYQYDDAARLVALFPGEDEPTTYEHDNGFVRVVRRGE
AVWKYERNDQGDVIRRTDPDGNATDYSYNKYGQLVGVWYPDHSCQTLVWNERGQLLEEQC
PNGGIKRYRYDDLGRKISQEDEHGALTQFEWNDVGRLVQIVLPSGDTRKYTYNPYGKITS
ERDELGNITRYEYADGLHLISRRLNADGSQVNYRYDNVRLLLTEIENEVGETYRLQYHSN
GLIQQETGFDGQRTAYVYDLNGNLQEKTEHGDDGSQLVTRYERDHAGRLVRKTLPDGNSV
NYAYDRQGNLLSVDDGNWFLAYEYDVQNRLTAEHQGWGTLRYGYDACGQLTNLRLPDNNR
LTFNHDKGGHLSTVELNGAVLTSHQFNADREQQRTQGKLLSTYQHDHQGRLFNQSIGDAE
GPVYRRHYDYDKSGNLTRLLDTRKGEHHYHYDPLSRLTRADHSQGEQERFGHDPAGNLLM
QNRPGPDIVAGNRLMIQGDHQYDYDAFGNLIRERRGKGHRLVTEYRYDCQHRLIGTKKPN
GQTASYRYDPFGRRISKTVGDVTTEFFWKGDTLIAEHHAERHRSYLYEPDSFRPLALLEG
FGPKETKAYHYQLDHLGTPQELTAADGEIVWSAHYRAYGEITRLDIGKIDNPLRFQGQYF
DQESGLHYNRHRYYHPDIGRYLTPDPVKLTGGINAYQYVPNPTGWVDPLGLSSCPGRDGC
KPKTEAALTTETTSVDKGEPRPPKGASTEYLYRGDHKNPDHVFQHGFKSKGNSNDLYLHA
LDSNNPPSNFISTSPSKITAIDFATRYGTRKGFLYALKKIPGRDINKELGKLAPFDNEAE
IAIQSKIDTENILGVTPMRRDGGYVGYSIPNPNRKVK