Protein Info for PFLU_RS17565 in Pseudomonas fluorescens SBW25

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1023 TIGR00594: DNA polymerase III, alpha subunit" amino acids 4 to 968 (965 residues), 539.5 bits, see alignment E=8.9e-166 PF02811: PHP" amino acids 5 to 98 (94 residues), 61 bits, see alignment E=4.8e-20 PF07733: DNA_pol3_alpha" amino acids 268 to 522 (255 residues), 290.9 bits, see alignment E=2.6e-90 PF17657: DNA_pol3_finger" amino acids 525 to 691 (167 residues), 202.7 bits, see alignment E=6.3e-64 PF14579: HHH_6" amino acids 765 to 853 (89 residues), 66.2 bits, see alignment E=6.2e-22 PF01336: tRNA_anti-codon" amino acids 933 to 1005 (73 residues), 33.2 bits, see alignment 1e-11

Best Hits

Swiss-Prot: 83% identical to DNAE2_PSEPF: Error-prone DNA polymerase (dnaE2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to pfs:PFLU3602)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXH4 at UniProt or InterPro

Protein Sequence (1023 amino acids)

>PFLU_RS17565 error-prone DNA polymerase (Pseudomonas fluorescens SBW25)
MSYAELHCLSNFSFQRGASSALELFERAKRQGYSALAITDECTLAGIVRAWQAAKAVELP
LIIGSEVRIENGPKLVLLVEDLSGYQHLCRLITLARRRAEKGSYRLLLEDFDQPLPGLLA
LWVAEDSDTQASIQWLRRTFAERLWLAVHLHCGQDDAHRLEQRLNLAASLRIPAVACGDV
HMHARGRRALQDTMTAIRHHVPVAEAGTRLHPNGERHLRSLDALAALYPHALLDETQAIA
RRCTFDLSQLRYHYPRELVPEGHDAESWLRAVTEAGIAQRWPQGVDAHTLQQIHKELALI
SELGYESYFLTVHDIVRFARSRSILCQGRGSAANSAVCFALGITEIDPSLTSMLFERFLS
KERNEPPDIDVDFEHERREEVLQYVFQRYGRTRAALTAVVSSYHAAGAVRDVAKALGLPP
DQVNALADCCGRWSDDAPPLERLREGGFDPQSPILRRVLTLTQQLIGFPRHLSQHPGGFV
ISEYPLDTLVPVENAAMAERTIIQWDKDDLDAVGLLKVDILALGMLSAIRRCFDLLRRHR
NRDFSLASLPKEDPATYAMISKADTIGVFQIESRAQMSMLPRLRPQTFYDLVIEVAIVRP
GPIQGGMVHPYLRRRNKEEETTYPSPELEVVLQRTLGIPLFQEQVMQIAMVAADYGPGEA
DQLRRSMAAWKRHGGLEPHQERLRTGMLKNGYSEEFAAQIFEQIKGFGSYGFPESHAASF
ALLTYASCWLKCHEPAAFACALINSWPMGFYSPDQILQDARRHRLQIRPVDVQASDWDCS
LEPIDGAQPAIRMGLRMIAGLREEEGRRIETARERGVFSDIADLDARAGLDARTQALLAD
AGALRALADNRHKARWEVAGVHKQLGLFAGLPSPDEAVVALPAPTVGEDLQADYATVGTT
LGPHPLALLRTELRKRRCRSSQELMLVEHGRNVSIAGLVTGRQRPGTASGVTFVTLEDEF
GNLNVVVWRDLAERQRQALVGSRLLKVDGRWEAVGEVRHLIAGRLTDLTPLLEGIPVRSR
DFH