Protein Info for PFLU_RS17285 in Pseudomonas fluorescens SBW25

Annotation: DotU family type IV/VI secretion system protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 244 transmembrane" amino acids 130 to 145 (16 residues), see Phobius details amino acids 197 to 221 (25 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 18 to 224 (207 residues), 210.3 bits, see alignment E=1.5e-66 PF09850: DotU" amino acids 20 to 219 (200 residues), 208.8 bits, see alignment E=3.5e-66

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to pfs:PFLU3545)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7U5 at UniProt or InterPro

Protein Sequence (244 amino acids)

>PFLU_RS17285 DotU family type IV/VI secretion system protein (Pseudomonas fluorescens SBW25)
MIYTAHLQGAPPFKMGSNILLAAAWELLLQVVQLKAGPGRESLQALNEQLSSGVNAFEAR
ALHQGVENSQVMSARYVLCSVIDEAVVTTSWGSRSDWSKTSLLSLFHNETFGGEKFFQLL
ERLSRDPVKHVAMLELMYVCLSLGFEGKYRVMERGRVQLETLHDAVYRQIRHVRGDRLPV
CISPETGRASQARIRLVSTTWVVICVVACLLAMYSGFAWMLGQERMTALQPFQSSVSVPS
RTPL