Protein Info for PFLU_RS16715 in Pseudomonas fluorescens SBW25

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 PF07992: Pyr_redox_2" amino acids 6 to 282 (277 residues), 161.3 bits, see alignment E=1.1e-50 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 7 to 800 (794 residues), 1022.7 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 148 to 217 (70 residues), 46 bits, see alignment E=1.8e-15 PF18267: Rubredoxin_C" amino acids 320 to 390 (71 residues), 39.1 bits, see alignment E=1.8e-13 PF04324: Fer2_BFD" amino acids 421 to 469 (49 residues), 31.9 bits, see alignment 4.2e-11 PF03460: NIR_SIR_ferr" amino acids 559 to 621 (63 residues), 46.8 bits, see alignment 6e-16 PF01077: NIR_SIR" amino acids 631 to 752 (122 residues), 78.5 bits, see alignment E=1.2e-25

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to pfs:PFLU3425)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KD76 at UniProt or InterPro

Protein Sequence (846 amino acids)

>PFLU_RS16715 nitrite reductase large subunit (Pseudomonas fluorescens SBW25)
MNSLKTLIVVGNGMVGHHCVAQLIERGVLDHYRLHVFSEEPMRAYDRVHLSEYFTGRDAE
SLALSDASLYQTPGVTLHLGVPVLEIDRARCEVITADGCVAYDKLVLATGSYPFVPPIEG
AEGDSRLVYRTLEDLDAIRKAAANARRGVVVGGGLLGLEAANALKSLGLEAHVVEFAPRL
MPVQLDDFGGLALKAQIERLGVGVHLSRATQSISAGEQYRYRMNFANDEFLETDLIVFSA
GIRAQDALARQAGLEIGPRGGVVINDEYLSCDPNIYAIGECASWNGSLFGLVAPGYQMAR
GVAALLCEQTAEPFVGADMSTKLKLLGVDVGSIGDAHAHTPGARSYQFIDEASASYRRLV
VDASGKHVIGAVLVGDNSYYDTLLQYMQNSIVLPSEPASLILPSSSGAPTLGPGALPESA
TVCSCHNVTKGSICSAIDSGCSDLGLLKSQTKACTGCGGCAGLLKQVFEHELIARGVSVD
KSLCEHFAHTRQELYALVRVEGIITFEELLAKHGRGHTGCDVCKPAVGSILASCWNQPIM
DASLVPLQDTNDTFMANMQKNGTYSVVPRIPGGEITADKLIVIGEVAKKYDLYTKITGGQ
RIDLFGAQLHELPDIWAELIAAGFETGHAYGKSTRTVKSCVGSTWCRYGVQDSVKMALLI
EDRYKGLRSPHKLKFAVSGCTRECAEAQSKDVGVIATEKGWNLYIAGNGGMRPRHAELFA
TDLDDATLIRYIDRFLMFYIRTADKLQRTSVWRESLEGGLDFLKDVILDDSLGLGAELEA
QMQQVVDRYECEWANALKDPEKLKRFRTFVNDKRPDPDIHFVQERGQRRPIMAAELNLIP
VIEEIA