Protein Info for PFLU_RS16415 in Pseudomonas fluorescens SBW25

Annotation: malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 transmembrane" amino acids 403 to 418 (16 residues), see Phobius details TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 21 to 543 (523 residues), 673.7 bits, see alignment E=7.9e-207 PF00128: Alpha-amylase" amino acids 125 to 213 (89 residues), 54.4 bits, see alignment E=1.5e-18 PF11941: DUF3459" amino acids 490 to 572 (83 residues), 62.7 bits, see alignment E=3.5e-21

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 100% identity to pfs:PFLU3365)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KD18 at UniProt or InterPro

Protein Sequence (581 amino acids)

>PFLU_RS16415 malto-oligosyltrehalose trehalohydrolase (Pseudomonas fluorescens SBW25)
MPLRTLETWPHGAIMLDAQHTRFALWAPDAFYVSVELENGKSVAMLPQAEGWFETEIACP
AGTRYRFNIDGEMDVPDPASRAQASDVHGWSVVVDSLAYQWRHSNWQGRPWHEAVIYELH
VGAMGGYAGIEKHLPRLAELGVTAIELMPLAQFPGDRNWGYDGVLPYAPQASYGTPEQLK
HLIDSAHEHGLAVILDVVYNHFGPDGNYLGQYAKGFFQEDVHTPWGAGIDFNRREVRDFF
LDNALMWLLEYRFDGLRLDAVHAIDNPEFLKELAHRVREQVDTGRHVWLMLENELNQAGL
LQHDFNAQWNDDFHNVLHVLLTGETDAYYSDFAQDPTAKLARCLGEGFIYQGHTTRHGHV
RGEPSGHLPPSAFVAFLQNHDQIGNRALGERLHQLCSPQALKAATALLLLSPMIPLMFMG
DEANASEPFLFFTDHHGELAEAVREGRRNEFKDFAAFQDPERRERIPDPNALPTFLQTTP
SFDENEHTQLYRQLLSLRHRHLVPHLPGSVALGAQVLADHAVSARWRLGNGSVLRIDLNL
SAVPLDHPATEPVLFQTPDNAGATLPPYSARVTLSPVGEHP