Protein Info for PFLU_RS16110 in Pseudomonas fluorescens SBW25

Annotation: benzaldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 24 to 480 (457 residues), 508.9 bits, E=5.8e-157

Best Hits

Swiss-Prot: 50% identical to XYLC_PSEPU: Benzaldehyde dehydrogenase [NAD(+)] (xylC) from Pseudomonas putida

KEGG orthology group: K00141, benzaldehyde dehydrogenase (NAD) [EC: 1.2.1.28] (inferred from 100% identity to pfs:PFLU3306)

MetaCyc: 50% identical to benzaldehyde dehydrogenase monomer (Pseudomonas putida mt-2)
Benzaldehyde dehydrogenase (NAD(+)). [EC: 1.2.1.28]; 1.2.1.28 [EC: 1.2.1.28]; 1.2.1.28 [EC: 1.2.1.28]; 1.2.1.28 [EC: 1.2.1.28]

Predicted SEED Role

"Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28)" in subsystem Phenylpropanoid compound degradation (EC 1.2.1.28)

MetaCyc Pathways

• 1,3-dimethylbenzene degradation to 3-methylbenzoate (1/3 steps found)
• 1,4-dimethylbenzene degradation to 4-methylbenzoate (1/3 steps found)
• 3-chlorotoluene degradation II (1/3 steps found)
• D-phenylglycine degradation (1/3 steps found)
• toluene degradation to benzoate (1/3 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• benzoate biosynthesis II (CoA-independent, non-β-oxidative) (1/4 steps found)
• mandelate degradation I (1/5 steps found)
• salicin biosynthesis (1/6 steps found)
• mandelate degradation to acetyl-CoA (7/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
• toluene degradation IV (aerobic) (via catechol) (2/13 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (13/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Biosynthesis of phenylpropanoids
• Toluene and xylene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.28

Use Curated BLAST to search for 1.2.1.28

Sequence Analysis Tools

See C3KCA6 at UniProt or InterPro

Protein Sequence (491 amino acids)

>PFLU_RS16110 benzaldehyde dehydrogenase (Pseudomonas fluorescens SBW25)
MSVSETTHLLQRALESECLFNGDWIPASGSVMPVIEPATGEPLMRCAMANAADIAVASRS
AALAQPAWAALGPRERAAVFRKAADLAQQCFAELALYVARETGGALFKGEHEVREAIVLL
HQAAGLLSQPHGLVLPSAAGRLSYARRQPHGVVGVISPFNFPLVLSLRSVAPALAAGNAV
VLKPDPQTPVSGGFLIARLFEEAGLPKGLLHVLPGAAPVGEALCRDPNVQMITFTGSTAA
GRKVAEVAGRHLKKVALELGGKNPLIILDDADLDLAASNAAWGAWLHQGQICMATGLILA
HESIAERLTRKLVDKARALTVGNAARGEAALGPLINPRQLQRVHEIVSDSLQAGARLEAG
GEYDQLFYQPTVLSGVRPGMRAFEDEVFGPVASVVSFATDEEAIELANRTEYGLSAAIIS
PSVGRAMAIGERLNCGLLHINDQTVADECINPFGGRGASGNGGSVGGPADWDEYSQWQWV
TVKNTPPVYPF