Protein Info for PFLU_RS15715 in Pseudomonas fluorescens SBW25

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1248 transmembrane" amino acids 1050 to 1069 (20 residues), see Phobius details PF00668: Condensation" amino acids 118 to 280 (163 residues), 42.8 bits, see alignment E=9e-15 PF00501: AMP-binding" amino acids 405 to 746 (342 residues), 242.8 bits, see alignment E=1.6e-75 TIGR01733: amino acid adenylation domain" amino acids 420 to 815 (396 residues), 366.1 bits, see alignment E=1e-113 PF13193: AMP-binding_C" amino acids 799 to 873 (75 residues), 34.5 bits, see alignment 9.6e-12 PF00550: PP-binding" amino acids 902 to 965 (64 residues), 47.2 bits, see alignment 6.5e-16 PF00975: Thioesterase" amino acids 986 to 1228 (243 residues), 101 bits, see alignment E=3.4e-32 PF00561: Abhydrolase_1" amino acids 994 to 1226 (233 residues), 27.9 bits, see alignment E=5.4e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3225)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC55 at UniProt or InterPro

Protein Sequence (1248 amino acids)

>PFLU_RS15715 non-ribosomal peptide synthetase (Pseudomonas fluorescens SBW25)
MSNYQAAFALSPQQRSQWLANAPPARLTLEVHGAAALAQLRERLAALSAVHDALRLQTRP
EPGLQVPLQIVAQSSAISLDVQLLAADVYRVTLQVPALAADRGTLLRLAQALGSAQAPIP
DDEAMTYTQYSAWIHDLQADEDAAQGRQYWAGLALGEQAATELLYREARSDQADTPLTTR
VMLDPALSAALHRHAAPAEQLLMTAWGVLLQRLSANDNAALTLNWVHDCRDDYEELADCW
GLFAKALPLPWQPAVERPFTQALATLQVLCEQAGEWQEYSLPVEAAPYGFQWSPGFDRAD
SAFKVLEIDAIPAGMELLLVAEAQGADGYRLTLCHQARHYSIQAAQTLLEQVQSLLLDAL
THPAKPLAELSLHAAGFAATLAARQQQVAPPFVSVPARFNACATSVAEHLALRDAGQHLS
YAELHARSNQLAHYLMAQGIGREDRVALYLDRSAQMVLAMLAVLKSGAAFVPLDVHQPTQ
RSLAILQQAQPRWVFTGAAGDPPALASMGSLDIRDTAVWQQAPMTDVAHDIKPGDAAYVL
FTSGSTGLPKGVIVEHAQLASYVSSVSSRLHLQAGERSAVVTSLAADLGYTLLFPTLLSG
GELHIIDKDTAMDAQAWAAWQAQYPIDHLKIVPSLLDAWLIHTQSAVVLPRKQLILGGET
CSRRLLQSVRAFAPALTVLNHYGPTETTVGVVMHKVDPACDYRRLPLDDRLDGVRLYLLD
EQQQLAAPGQNAELYIAGPQLARGYLDAQHDDGRFIQRNGERLYRTGDRARYRHDASLEI
TGRTDRQVKVRGFRVELDEIQAQLASLPGVAQAAVECLPKGELGQQLFAFLTLAPGHTVT
VAHLHGQAQDRLPDYMLPNVRIVEALPLMGNGKLDRKTLQQWADKVLDNVGGALPRTPLE
ALLAEVWAQVLGLERVGIEDDFFALGGHSLAAVSLASRLQTVLSAPVTVNAVFNAPSVAA
FAALVQAQFTLSPLVRLSAAGPGINLFCFHPSTGHVQDYRALLPHLPDWQVWGLQAAYLG
DDSTALNGNIDSLAALYVEQLRQQQPHGPYHLLGFSLGGLLAITAAAQLEKCGERVAFLG
ILDSQYQRQAEEDSVETLLDSAAQALTVESQARLRNLPAPVSTALIARLATLPAAERLPA
LAQWAHQQGLQLEGDSWEHLHTRLGYQQQTQQLLASFQPPRLSCPVHIWWADTTLQAADF
TDPQWEQLSSGPWFRDVVQAQHLNILEQPTVHQQLVVGLAALATDGAV