Protein Info for PFLU_RS15675 in Pseudomonas fluorescens SBW25
Annotation: diaminobutyrate--2-oxoglutarate transaminase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to RHBA_RHIME: Diaminobutyrate--2-oxoglutarate aminotransferase (rhbA) from Rhizobium meliloti (strain 1021)
KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3217)MetaCyc: 64% identical to L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase monomer (Pseudomonas aeruginosa PAO1)
Diaminobutyrate--2-oxoglutarate transaminase. [EC: 2.6.1.76]
Predicted SEED Role
"Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76)" (EC 2.6.1.76)
MetaCyc Pathways
- pyoverdine I biosynthesis (9/11 steps found)
- norspermidine biosynthesis (4/6 steps found)
- ectoine biosynthesis (3/5 steps found)
- superpathway of polyamine biosynthesis III (5/8 steps found)
- ectoine degradation (1/4 steps found)
- rhizobactin 1021 biosynthesis (1/7 steps found)
- baumannoferrin biosynthesis (1/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.76
Use Curated BLAST to search for 2.6.1.76
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3KC47 at UniProt or InterPro
Protein Sequence (471 amino acids)
>PFLU_RS15675 diaminobutyrate--2-oxoglutarate transaminase family protein (Pseudomonas fluorescens SBW25) MSAFSNATHGGAPQLRLAHTGLSQPYCLDLTPMLERQQQQESNARSYPRRIPLVLERAYG IYVQDSRGQVFVDCLAGAGTLALGHNHPVVIEAITQVMAAGVPMHTLDLMTPVKDAFVQE VFGCLPADFARNARIQFCGPSGADAVEAALKLTRTATGRHSVLAFEGAYHGMTLGTLAIS GNLSPKNALGALMPGVQRLPYPHDYRCPFGVAGDQGVTLNVRYLEHLLNDPESGVTLPAA MILEPIQGEGGVIAAPDRWLQELRRLTRAHGIPLIVDEIQCGIARSGRMFGFEQSGITPD VITLSKAIGGGLPLSVMVYNDALDVWQPGAHAGTFRGNQLAMAAGTATLRFIRDNDLVAH AETVGAHLRKQLQALQSEFTWVGDVRGRGLMLGMEIVDPCGVPDVQGHPPVDTARAKAFQ QACLKHGLIVELGGRHGATVRFLPPLIITEQEIDFVAQILFQAAHSITTQP