Protein Info for PFLU_RS15575 in Pseudomonas fluorescens SBW25

Annotation: fumarylacetoacetate hydrolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 PF01557: FAA_hydrolase" amino acids 76 to 279 (204 residues), 219.6 bits, see alignment E=2.1e-69

Best Hits

Swiss-Prot: 47% identical to UGL_BURA4: Ureidoglycolate lyase (BamMC406_5393) from Burkholderia ambifaria (strain MC40-6)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3196)

Predicted SEED Role

"Fumarylacetoacetate hydrolase family protein" in subsystem Gentisare degradation or Salicylate and gentisate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC26 at UniProt or InterPro

Protein Sequence (289 amino acids)

>PFLU_RS15575 fumarylacetoacetate hydrolase family protein (Pseudomonas fluorescens SBW25)
MRLLRVGAPGCERPCAVGVDGSVRDLSEWVNDWTGAALAVAAMETLSRRFTLEWAQLPQV
HIEHERIGPALRPGQILSIGLNYHRHAREAGMAVPEEPIVSSKSVHALGGPFDDLVLPVD
GDKTDWEVELGVVIGRRAQYLATPADAAACILGYCTANDLSERSWLLERGGQWIKGKSFD
GFAPLGPYLVTADEVPSPQALELICRVNGEVMQRDSSEDMIFDVPHLIHYLSRFMTLHPG
DVILTGSPGGMALGRPDQPFLRDGDVVEAEVVGLGAQRQVCRAFYKEVT