Protein Info for PFLU_RS15455 in Pseudomonas fluorescens SBW25

Annotation: SDR family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 7 to 198 (192 residues), 189.7 bits, E=6.2e-60
• PF08659: KR
  • amino acids 8 to 172 (165 residues), 42.1 bits, E=1.3e-14
• PF13561: adh_short_C2
  • amino acids 13 to 227 (215 residues), 152.3 bits, E=2.6e-48

Best Hits

Swiss-Prot: 44% identical to Y432_LISMO: Uncharacterized oxidoreductase Lmo0432 (lmo0432) from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3171)

MetaCyc: 34% identical to NAD-dependent bile acid 3beta-dehydrogenase (Eggerthella lenta DSM 2243)
RXN-11472 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]

Predicted SEED Role

"Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140)" in subsystem D-Sorbitol(D-Glucitol) and L-Sorbose Utilization (EC 1.1.1.140)

MetaCyc Pathways

• D-sorbitol degradation II (1/1 steps found)
• L-sorbose degradation (1/2 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• iso-bile acids biosynthesis I (2/4 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• bile acids 3-O-epimerization (1/4 steps found)
• bile acids epimerization (1/12 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.140 or 1.1.1.391

Sequence Analysis Tools

See C3KBR2 at UniProt or InterPro

Protein Sequence (247 amino acids)

>PFLU_RS15455 SDR family oxidoreductase (Pseudomonas fluorescens SBW25)
MNYLQNKTAIITGASSGLGAASARALSEKGVRIVAAALDQQGLDAIVSELKGKGGEAVGR
VSDVTNPEDMKALAQFAQDSFGSVDILINNAGLMLFSNWVDLATDDWEKMINVNIKGYLH
GIAAVLPFMLKQKSGQILNMDSVAGHQVGPAAGIYSATKFFVQAMTESMRKELGVQHGIR
VNTVSPGVINTGWADKVTDPAGRKAAQELNKIAISPDDIGRAVVYALNQPENVTVNDLII
SPTRQDW