Protein Info for PFLU_RS15340 in Pseudomonas fluorescens SBW25

Annotation: matrixin family metalloprotease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00413: Peptidase_M10" amino acids 97 to 197 (101 residues), 32.8 bits, see alignment E=1.6e-11 PF13583: Reprolysin_4" amino acids 164 to 240 (77 residues), 30.1 bits, see alignment E=9.4e-11 PF08548: Peptidase_M10_C" amino acids 259 to 476 (218 residues), 258 bits, see alignment E=1.6e-80 PF00353: HemolysinCabind" amino acids 342 to 369 (28 residues), 23.4 bits, see alignment (E = 1.2e-08) amino acids 352 to 386 (35 residues), 40.8 bits, see alignment 4.3e-14

Best Hits

Swiss-Prot: 98% identical to APRA_PSEPA: Metallopeptidase AprA (aprA) from Pseudomonas panacis

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3146)

Predicted SEED Role

"Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog" in subsystem Protein secretion by ABC-type exporters (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBF4 at UniProt or InterPro

Protein Sequence (477 amino acids)

>PFLU_RS15340 matrixin family metalloprotease (Pseudomonas fluorescens SBW25)
MSKVKDKAIVSAAQASTAYSQIDSFSHLYDRGGNLTINGKPSYTVDQAATQLLRDGATYR
DFDGNGKIDLTYTFLTSASSSTMNKHGISGFSQFNAQQKAQAVLAMQSWADVANITFTEK
ASGGDAHMTFGNYSGGQDGAAAFAYLPGTGAGYDGTSWYLTNNSYTPNKTPDLNNYGRQT
LTHEIGHTLGLAHPGDYNAGEGAPTYNDATYGQDTRGYSLMSYWSESNTNQNFSKGGVEA
YASGPLIDDIAAIQKLYGANYNTRAGDTTYGFNSNTGRDFLSATSNADKLVFSVWDGGGN
DTLDFSGFTQNQKINLNEASFSDVGGLVGNVSIAKGVTIENAFGGAGNDLIIGNNAANII
KGGAGNDLIYGGGGADQLWGGAGNDTFVFGASSDSKPGASDKIFDFTSGSDKIDLSGITK
GAGLTFVNAFTGHAGDAVLTYAAGTNLGTLAVDFSGHGVADFLVTTVGQAAVSDIVA