Protein Info for PFLU_RS15330 in Pseudomonas fluorescens SBW25

Annotation: type I secretion system permease/ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 58 to 84 (27 residues), see Phobius details amino acids 128 to 173 (46 residues), see Phobius details amino acids 249 to 281 (33 residues), see Phobius details TIGR01842: type I secretion system ATPase" amino acids 16 to 556 (541 residues), 784.8 bits, see alignment E=2.1e-240 PF00664: ABC_membrane" amino acids 24 to 281 (258 residues), 41.2 bits, see alignment E=1.7e-14 PF00005: ABC_tran" amino acids 347 to 493 (147 residues), 106.8 bits, see alignment E=1.4e-34

Best Hits

Swiss-Prot: 59% identical to APRD_PSEAE: Alkaline protease secretion ATP-binding protein AprD (aprD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12536, ATP-binding cassette, subfamily C, bacterial HasD (inferred from 100% identity to pfs:PFLU3144)

Predicted SEED Role

"ABC-type protease exporter, ATP-binding component PrtD/AprD" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBF2 at UniProt or InterPro

Protein Sequence (583 amino acids)

>PFLU_RS15330 type I secretion system permease/ATPase (Pseudomonas fluorescens SBW25)
MAKSHAVAPLFKALGEYKSILISVGCFTALINLLMLVPSIYMLQVYDRVLSSQNETTLVM
LTLMVVGFFAFIGLLEVIRSFIVIRIGSQLERRFNLRVYKAAFERNLQRGQGHAGQSLGD
LTHIRQFITGPALFAFFDAPWFPLYLFVIFLFNVWLGVLATAGAVLLIGLACLNEYLTKK
PLGEASGFSQQSTQLATSHLHNAETIQAMGMLGALRSRWFAVHSQFLGLQNTASDTGSVI
TSLSKSLRLCLQSLVLGLGALLVIKGDMTAGMMIAGSILMGRVLSPIDQLIAVWKQWSSA
KLAYQRLDDLLREFPPDVEQMKLPAPKGQVSFEQVSAGPPGRRVATLHQVSFNLGAGEVL
GVLGASGSGKSTLARVLVGVWPTLAGTVRLDGADIHRWDRDDLGPHIGYLPQDIELFSGS
IADNIARFREADPDQVVRAAQQAGVHELILRLPQGYDTVLGDNGGGLSGGQKQRVALARA
LYGGPRLIVLDEPNSNLDTVGEAALASAIVQMKAQGSSVVLVTHRSSALAQADKLLVLND
GHLQAFGPSQDVLRALSGQQEAPKEKPGVSFSRQYQAGRNPGA