Protein Info for PFLU_RS15320 in Pseudomonas fluorescens SBW25

Annotation: TolC family outer membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 23 to 439 (417 residues), 382.3 bits, see alignment E=1.6e-118 PF02321: OEP" amino acids 24 to 214 (191 residues), 82.3 bits, see alignment E=2.1e-27 amino acids 241 to 426 (186 residues), 105.6 bits, see alignment E=1.4e-34

Best Hits

Swiss-Prot: 52% identical to APRF_PSEAE: Alkaline protease secretion protein AprF (aprF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3142)

Predicted SEED Role

"ABC-type protease exporter, outer membrane component PrtF/AprF" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBF0 at UniProt or InterPro

Protein Sequence (442 amino acids)

>PFLU_RS15320 TolC family outer membrane protein (Pseudomonas fluorescens SBW25)
MKHVFIALLLTCANAQAAMGPFDVYEQALRNDPVFLGALKERDAGLENRAIGRAGLLPKL
SYTYNKGRNNSQATLPDGRGGNYHDDRNYNSYGSTFSLQQPLFDYEAYANYRKGVAQALF
ADESFRDKSQALLVRVLSYYTQALFAQDQIDIARAKKKAFEQQFQQNRHLFQQGEGTRTD
ILEAESRYELATAEEIQALDEQDASLRELGALIGVQSVNIDDLAPLNQGFAAFALTPANY
DTWHELAISNNPTLASQRQALEVARYEVERNRAGHLPKVTAYASSRQQESDSGNTYNQRY
DTNTIGVEVSVPLYAGGGISASTRQASRAMEQAEYELEGKTRETLIELRRQFSACLSGVS
KLRAYQKALASAEALVVSTKQSILGGERVNLDALNAEQQLYSTRRDLAQARYDYLMAWTK
LHYYAGNLRDTDLAKVDEAFGR