Protein Info for PFLU_RS15305 in Pseudomonas fluorescens SBW25

Annotation: VRR-NUC domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 PF21315: FAN1_HTH" amino acids 9 to 90 (82 residues), 130.3 bits, see alignment E=2.9e-42 PF18081: FANC_SAP" amino acids 95 to 144 (50 residues), 44.6 bits, see alignment 2e-15 PF08774: VRR_NUC" amino acids 432 to 544 (113 residues), 126.1 bits, see alignment E=1.1e-40

Best Hits

Swiss-Prot: 60% identical to FAN1_PSEAE: Fanconi-associated nuclease 1 homolog (fan1) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3139)

Predicted SEED Role

"Hypothetical protein, restriction endonuclease-like VRR-NUC domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBE7 at UniProt or InterPro

Protein Sequence (548 amino acids)

>PFLU_RS15305 VRR-NUC domain-containing protein (Pseudomonas fluorescens SBW25)
MANPLEEPLYYLHNFRQVLHWLGQRYADLLDPDEEHFIQQFDRLPQASQALLVRMVMRKG
VHFRAGKLNYVEIGCTQAAAGPLLELGWVDDECLLAFDALFALLQKGEILAAFKPWIEQP
KAKKTDWLEGLSAHFTDNRCFAHWCPDLADTLYSLTVMDLCDRLRLMFFGNLHQDWSEFV
LADLGIYTYEKVEFCAESRGLRSRDDVQGFLFLHQCQQAFETGAALEEVLAQVATLNTDN
PWLEKRRAKLLFQVGQYCERTAELALAEQIYRACAYPGARSRLIRVLERQEHYVQAMALA
NAAQQAPESAAEQQHLLRVVPRLRRKLGEPALPKVKPRPVTRLDLALALPDPLMSVEYCV
QAHLSEPDGPVHYVENGLINSLFGLLCWDVIFAPLPGSFFHPFQRGPADLHSEDFHLRRA
PLFAACFEQLQDERYKTTIRQRYSEKWGIQSPFVFWNLLSAELLEQALACLPAEHLRYWF
ERLLLDIRANRAGMPDLIQFWPAQKTYRMIEVKGPGDRLQDNQLRWLEFCGEYQMPVTVC
YVRWAASA