Protein Info for PFLU_RS15170 in Pseudomonas fluorescens SBW25

Annotation: U32 family peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 PF01136: Peptidase_U32" amino acids 79 to 304 (226 residues), 237.8 bits, see alignment E=9.9e-75 PF12392: DUF3656" amino acids 399 to 513 (115 residues), 62.8 bits, see alignment E=4.7e-21

Best Hits

Swiss-Prot: 69% identical to RLHA_ECOLI: 23S rRNA 5-hydroxycytidine C2501 synthase (rlhA) from Escherichia coli (strain K12)

KEGG orthology group: K08303, putative protease [EC: 3.4.-.-] (inferred from 100% identity to pfs:PFLU3110)

MetaCyc: 69% identical to 23S rRNA 5-hydroxycytidine C2501 synthase (Escherichia coli K-12 substr. MG1655)
RXN-21990

Predicted SEED Role

"Putative collagenase"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBB9 at UniProt or InterPro

Protein Sequence (659 amino acids)

>PFLU_RS15170 U32 family peptidase (Pseudomonas fluorescens SBW25)
MSLPKHHLELLSPARDVAIAREAILHGADAIYIGGPSFGARHNACNDVSDIASLVEFARR
YHARVFTTINTILHDNELEPARKLIHQLYDAGVDALIVQDLGVMELDIPPIELHASTQTD
IRTLARAKFLDQAGFSQLVLARELNLQEIRAIADETEAAVEFFIHGALCVAFSGQCNISH
AQNGRSANRGDCSQACRLPYTLKDDQGRVVAFEKHLLSMKDNNQSANLRALVEAGVRSFK
IEGRYKDMGYVKNITAYYRQRLDEILEDRPDLARASSGRTAHFFLPDPEKTFHRGSTDYF
VSDRKIDIGAFDTPTFTGLPVGVVEKAGKRDLQVVTHEPLSNGDGLNVLVKREVVGFRAN
IAEPKGEFEEDGEKRYRYRVEPNEMPAGLHQLRPNHPLNRNLDHNWQQALLKTSSERRIG
LSWVARLREEQLEVVATSEEGISASVTLRGPFGVANKPEQALDTLRDLLGQLGTTEYHAT
NIQLDAPQAFFIPNSQLKALRREVIEALTTARIAAHPRGGRKAETSPPPVYPDAHLSFLA
NVYNQKARDFYHRHGVKLIDAAFEAHEETGEVPVMITKHCLRFSFNLCPKQAKGVTGVKT
KVAPMQLIHGDEVLTLKFDCKPCEMHVVGKIKGHILGLPQPGSVVEHFNPENIIFQGTH