Protein Info for PFLU_RS15105 in Pseudomonas fluorescens SBW25

Annotation: aspartate aminotransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00202: Aminotran_3
  • amino acids 40 to 453 (414 residues), 332.2 bits, E=2e-103

Best Hits

KEGG orthology group: K00823, 4-aminobutyrate aminotransferase [EC: 2.6.1.19] (inferred from 94% identity to pba:PSEBR_a3056)

Predicted SEED Role

"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt II (3/4 steps found)
• superpathway of L-arginine and L-ornithine degradation (9/13 steps found)
• GABA shunt I (2/4 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (7/11 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Butanoate metabolism
• Glutamate metabolism
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19, 2.6.1.81

Use Curated BLAST to search for 2.6.1.19 or 2.6.1.81

Sequence Analysis Tools

See C3KBA6 at UniProt or InterPro

Protein Sequence (458 amino acids)

>PFLU_RS15105 aspartate aminotransferase family protein (Pseudomonas fluorescens SBW25)
MSEAKLQSLHFADAPQIISGELPGPKTREALALSARTESMARGGGRMPVAMDRAFGATFK
DPDGNTYIDLSAGVGVSSVGRCHPKVVQAIREQSEVLMHALEINSTRRTELAAKLSEIAP
EGLRGDCITFFTQSGSDALEAAIKFAKRITGRHQIIAFHGGYHGVWNASGSLTTGTAYRK
GFGAQMGGVIHAPYPYAYRFPFDTTHKSAEQIAGEYIDYLLNTPYTAADDVAAVIVEPVQ
GEGGYVPPSPEFLQLLRKACDRSGTLLIVDEVQSGAGRTGKMWAVEHSGVKPDMLTFGKG
IGSDLPMAGLIMRSDLAAKIPDGSMPNTFAANSLSAAVALTNISILQDPELDLLNRAHTL
GLEAHAHIRGFNSPLVGEVRGRGLMIGIELIEDPLTKAPLAGEKIGQLMGYLLNHGVLMI
PCGRYSNVMRVMPSLTISRELFFKALDIFGDALASLKA