Protein Info for PFLU_RS14975 in Pseudomonas fluorescens SBW25

Annotation: FAD-binding oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 20 to 412 (393 residues), 235.8 bits, E=3.2e-73
• PF00890: FAD_binding_2
  • amino acids 20 to 219 (200 residues), 35.1 bits, E=2.8e-12
• PF13450: NAD_binding_8
  • amino acids 22 to 50 (29 residues), 26.4 bits, see alignment (E = 2.1e-09)

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3070)

MetaCyc: 80% identical to tyramine oxidase subunit A (Pseudomonas putida U)
Monoamine oxidase. [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

• phenylethylamine degradation I (2/2 steps found)
• putrescine degradation III (3/4 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (2/4 steps found)
• salidroside biosynthesis (2/4 steps found)
• serotonin degradation (4/7 steps found)
• L-tryptophan degradation VI (via tryptamine) (1/3 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• dopamine degradation (2/5 steps found)
• phenylethanol biosynthesis (2/5 steps found)
• aromatic biogenic amine degradation (bacteria) (4/8 steps found)
• melatonin degradation II (1/4 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• superpathway of melatonin degradation (1/11 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Arginine and proline metabolism
• Drug metabolism - cytochrome P450
• Glycine, serine and threonine metabolism
• Histidine metabolism
• Nitrogen metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.4, 1.4.99.1

Use Curated BLAST to search for 1.4.3.4 or 1.4.99.1

Sequence Analysis Tools

See C3KB80 at UniProt or InterPro

Protein Sequence (430 amino acids)

>PFLU_RS14975 FAD-binding oxidoreductase (Pseudomonas fluorescens SBW25)
MAPTIAPVTTSTVFPPAASVVIIGGGIIGLTAALTLAERNISVVVLEKGRIAAEQSSRNL
GWVRKTSRHAADIPLALAADRLWAQMPERVGADVGYRQEGIMFVGRTSAQMAMHESWLKS
VEHLSLDSRLLSKREIDARVPGGVGDWAGGIFTPSDARAEPTLASSAIAKAAMAMGVVII
EQCAVRTLQMSAGAVSGVVTEKGEIRCDHVLLAGGMWSRRFLGNLGVSLPTLPLTCSVLR
THPMDGPTEIAVGAPDFSFRKHKDGGFIITQRGKLDAFLTLDHLLLAKQYMPQFRAQRSV
LNVSLGKPFINDLALARRWRADSVSPFERVRVQDPEANPRLNNDAMNNLIAAWPVFQQAR
IAHAWAGTIDVTPDSNPVIGPVAQIPGLTVATGFSGHGFGTSPAAGHLAADLVSGHTPII
DPSPYRFDRF