Protein Info for PFLU_RS14825 in Pseudomonas fluorescens SBW25

Annotation: bifunctional UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase ArnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF00551: Formyl_trans_N" amino acids 27 to 178 (152 residues), 91.2 bits, see alignment E=1.5e-29 PF02911: Formyl_trans_C" amino acids 205 to 283 (79 residues), 60.7 bits, see alignment E=2.7e-20 PF01370: Epimerase" amino acids 321 to 568 (248 residues), 148.6 bits, see alignment E=4.2e-47 PF16363: GDP_Man_Dehyd" amino acids 322 to 645 (324 residues), 86.3 bits, see alignment E=5.6e-28

Best Hits

Swiss-Prot: 100% identical to ARNA_PSEFS: Bifunctional polymyxin resistance protein ArnA (arnA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 100% identity to pfs:PFLU3041)

MetaCyc: 70% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) / UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAD2 at UniProt or InterPro

Protein Sequence (663 amino acids)

>PFLU_RS14825 bifunctional UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase ArnA (Pseudomonas fluorescens SBW25)
MSSKAVVFAYHDIGCAGIEALLNTGYDIAAVFTHADDPKENNFYGSVAQLCARNGIPVHA
PEDANHPLWIERIAKLNPDYLFSFYYRNLLSEPLLATARKGAFNLHGSLLPKYRGRAPAN
WVLVNGETETGVTLHRMVKRADAGAILAQQKVIIERSDTGLTLHAKLRDAASNLLRDALP
QLAQGKLAETAQDESQATYFGRRTAADGKLEWKKPAEELFNLVRAVTQPYPGAFCAVGEH
KLVVWQADVVKGNEGLAPGRVISVNPLRIACGEDSLVIKFGQRNDNGLYLAGPSLANELG
LVDGSVLRGAESGRKPRRTRVLILGVNGFIGNHLSERLLRDDRYEVYGLDIGSDAIERLR
SHPNFHYVEGDISIHTEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKLV
RYCVKYNKRVIFPSTSEVYGMCQDQYFDEDTSNLVVGPVNKQRWIYSVSKQLLDRVIWAY
GAKGLNFTLFRPFNWMGPRLDRLDSARIGSSRAITQLILNLVEGTPIRLFDGGEQKRCFT
DIADGIEALARIIDNDNDACNGQIINIGNPENEASIRQLGEELLRQFEAHPLRGNFPPFA
GFRDVESKAFYGTGYQDVAHRKPSIENAKRLLNWEPTVEMSETIGNTLDFFLREAMLEIA
DKK