Protein Info for PFLU_RS14635 in Pseudomonas fluorescens SBW25

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 22 to 39 (18 residues), see Phobius details amino acids 61 to 82 (22 residues), see Phobius details amino acids 92 to 111 (20 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 150 to 173 (24 residues), see Phobius details amino acids 186 to 208 (23 residues), see Phobius details amino acids 254 to 275 (22 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 320 to 339 (20 residues), see Phobius details amino acids 345 to 365 (21 residues), see Phobius details amino acids 375 to 399 (25 residues), see Phobius details amino acids 411 to 430 (20 residues), see Phobius details PF07690: MFS_1" amino acids 30 to 396 (367 residues), 165.9 bits, see alignment E=6.4e-53

Best Hits

Swiss-Prot: 46% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3002)

Predicted SEED Role

"D-glycerate transporter (predicted)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KA93 at UniProt or InterPro

Protein Sequence (436 amino acids)

>PFLU_RS14635 MFS transporter (Pseudomonas fluorescens SBW25)
MSQSAAVTLATDDDKNAIYKRVTLRLIPFIFICYLFNYLDRVNVGFAKLQMLDALKFSET
VYGLGAGIFFIGYVLCGVPSNLALTKFGPRRWIALMMIVWGTLSTCLLFVTTPTHFYTLR
LFTGAAEAGFFPGVVLYLSQWFPTFRRGRIMALFMSAIPVSGLLGSPFSGWILNHFAAGQ
GGLAGWQWMFLLQGIPTVILGALAYFLLSDSFAHAKWLKPHERAVLEADQATDLANKPKT
TTDSLAEVFKNPAIWAFGLIYFCIQSGVYAINFWLPSIIKNLGFSDNLVIGWLSAIPYLL
AAVFMLLVGRSADLRKERRWHLVVPMLMGAIGLVIAVNFATTPAIAILGLTIATMGALTG
LPMFWPVPTAMLSAGAAAGGLALINSMGQMAGFLSPYIVGFVKDATGSTDVALYLLAAVI
VAGSVLALRMTRTLKV