Protein Info for PFLU_RS14255 in Pseudomonas fluorescens SBW25

Annotation: TolC family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 11 to 460 (450 residues), 341.7 bits, see alignment E=3.5e-106 PF02321: OEP" amino acids 61 to 249 (189 residues), 61.5 bits, see alignment E=4.8e-21 amino acids 278 to 458 (181 residues), 76 bits, see alignment E=1.8e-25

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2929)

Predicted SEED Role

"Outer membrane protein OprN"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KB17 at UniProt or InterPro

Protein Sequence (471 amino acids)

>PFLU_RS14255 TolC family protein (Pseudomonas fluorescens SBW25)
MSVKVFLPSLLVLALSACAVGPDYKTQTPEAANITAAADAKQYDHAKFEGIWWQQFEDPT
LNQLVTQSLQGNRDLRVAFARLRAARAIRDDASNDAMPTITSRASSDQGKGQIPGQTTRR
VKTERYDLGLDMAWELDLFGRIQRNLEATDADQQAAEADLYQLQVTLIAELVDAYGQLRG
AQLRERIALDNLKNQQESRTITVSLRDAGVGDQLDVERADARLAAVEASVPQLQAEQVRE
RNRIATLLGQRPDKLSVDLGPKDLPAIAKALPIGDPGQLLQRRPDILSAERKLAAATARI
GVAKADLFPRVSLSGFLGFTAGRGSQIGSSAANAWALGPSITWAAFDLGSVRARLRGADA
EADGALASYEQQVLLALEESENAFSDYGKRQQRLVSLIRQSESSRAAADLAAIRYREGTV
DFLVLLDAQRERLAAEDSQAQAEVDLYRGIVAIYKALGGGWKPDTVVATAK