Protein Info for PFLU_RS14225 in Pseudomonas fluorescens SBW25

Annotation: deferrochelatase/peroxidase EfeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details TIGR01412: Tat-translocated enzyme" amino acids 8 to 423 (416 residues), 490.5 bits, see alignment E=3.9e-151 TIGR01413: Dyp-type peroxidase family" amino acids 62 to 411 (350 residues), 292 bits, see alignment E=6.2e-91 PF04261: Dyp_perox_N" amino acids 62 to 212 (151 residues), 123.6 bits, see alignment E=6.7e-40 PF20628: Dyp_perox_C" amino acids 229 to 411 (183 residues), 170.7 bits, see alignment E=2.3e-54

Best Hits

Swiss-Prot: 58% identical to EFEB_SHISS: Deferrochelatase/peroxidase EfeB (efeB) from Shigella sonnei (strain Ss046)

KEGG orthology group: K07223, putative iron-dependent peroxidase (inferred from 100% identity to pfs:PFLU2919)

MetaCyc: 58% identical to heme-containing peroxidase/deferrochelatase (Escherichia coli K-12 substr. MG1655)
PROTOHEMEFERROCHELAT-RXN [EC: 4.98.1.1]

Predicted SEED Role

"Ferrous iron transport peroxidase EfeB"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.98.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KB07 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PFLU_RS14225 deferrochelatase/peroxidase EfeB (Pseudomonas fluorescens SBW25)
MSDSAQFDLQRRRVLLGMAATGAAIAGSSLTCPAMAAAAEQVTTAPRSDKTQDHHDFFGT
HQAGIVTPRPACGMLVAFDVLASDREDLERLFRTLNERIAFLMTGGTVPQVDPKLPPTDS
GILGPVVTPDNLTITVSVGESLFDERFGLAAVKPKRLSRMVGFPNDALEPAQCHGDLSLQ
FSSNTTDTNIHALRDIVKNLPDLLLVRWKQEGSVPPQAPAKPGEPAQSARNFLGFRDGSA
NPNSNDDKAMNQIVWVQPGSDEPAWAANGSYQAVRIIRNFVERWDRTPLQEQESIIGRIK
PTGAPMDGDKESQVPDYSKDPEGKLTKLDAHIRLANPRTPQTQANLILRRPFNYSNGVNK
NGQLDMGLLFICYQADLEKGFISVQTRLNGEPLEEYLKPVGGGYFFTLPGVTGPKDFLGR
TLLAATHPHTTAPT