Protein Info for PFLU_RS13685 in Pseudomonas fluorescens SBW25

Annotation: gamma-glutamyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 35 to 541 (507 residues), 586.8 bits, see alignment E=1.7e-180 PF01019: G_glu_transpept" amino acids 50 to 567 (518 residues), 585.6 bits, see alignment E=5.1e-180

Best Hits

Swiss-Prot: 81% identical to GGT_PSEUA: Glutathione hydrolase proenzyme (ggt) from Pseudomonas sp. (strain A14)

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to pfs:PFLU2805)

MetaCyc: 53% identical to glutathione hydrolase proenzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9Y3 at UniProt or InterPro

Protein Sequence (574 amino acids)

>PFLU_RS13685 gamma-glutamyltransferase (Pseudomonas fluorescens SBW25)
MKYQPFSRTLIATALVLTVSGVHAASQAPVAGENGMVVTAQHLATHVGVDVLKAGGNAVD
AAVAVGYALAVVYPAAGNLGGGGFMTVQLADGRKTFLDFREKAPLAATADMYLDKDGNVI
EGLSAKGHLAVGVPGTVSGMELALSKYGTLKRAQVIAPAIKLAENGFALEQGDIDLLHTA
TGEFEKDKDMRAIFLHNGQPLQVGQKLVQKDLAKTLKEISAKGTDGFYKGWVAKALVDSS
QAGKGIITQADLDKYKTRELAPIECDYRGYHVVSAPPPSSGGVVICQIMNILEGYPMADL
GYHSAQGLHYQIEAMRHAYVDRNSYLGDPDFVKNPIEHLLDKNYATKLRDAIEPQKAGDS
QAIKPGVSPHEGNNTTHYSIVDKWGNAVSVTYTLNDWFGAGVMASKTGVILNDEMDDFTV
KVGVPNMYGLVQGEANAIAPGKAPLSSMSPTIVTKDGKAVMVVGTPGGSRIITATLLTIL
NVIDYKMNIQEAVDAPRFHQQWMPETTNLETFAVSPDTQKILESWGHKFAGPQDANHLAA
ILVGAPSLGGKPVGNNRFYGANDPRRNTGLSLGY