Protein Info for PFLU_RS13675 in Pseudomonas fluorescens SBW25

Annotation: molybdopterin-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1175 transmembrane" amino acids 249 to 266 (18 residues), see Phobius details amino acids 758 to 777 (20 residues), see Phobius details PF20256: MoCoBD_2" amino acids 44 to 186 (143 residues), 78.7 bits, see alignment E=1.1e-25 amino acids 579 to 685 (107 residues), 59.1 bits, see alignment E=1e-19 PF02738: MoCoBD_1" amino acids 311 to 548 (238 residues), 104.6 bits, see alignment E=9.7e-34 PF00034: Cytochrom_C" amino acids 798 to 896 (99 residues), 32 bits, see alignment E=5.4e-11 amino acids 1067 to 1148 (82 residues), 33.1 bits, see alignment 2.4e-11 PF13442: Cytochrome_CBB3" amino acids 1067 to 1147 (81 residues), 40.2 bits, see alignment 7.2e-14

Best Hits

Swiss-Prot: 76% identical to NICB_PSEPK: Nicotinate dehydrogenase subunit B (nicB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2803)

MetaCyc: 76% identical to nicotinate hydroxylase large subunit (Pseudomonas putida KT2440)
NICOTINATE-DEHYDROGENASE-RXN [EC: 1.17.2.1]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.17.2.1 or 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9Y1 at UniProt or InterPro

Protein Sequence (1175 amino acids)

>PFLU_RS13675 molybdopterin-dependent oxidoreductase (Pseudomonas fluorescens SBW25)
MTDTPLSRDQWLAKAGVLLIVDDVLPPSGPVAKGGTPTVKPKELGLFIAVSDDGRVYAFN
GHVDLGTGIRTSLAQIVAEELDLTMEQVKMVLGDTERAPNQGATIASATLQISAVPLRHA
AAEARRFLLARAAGRWGVSTASLKVDAGVIQAADGRTTSYFELVSGQHDQLRISGDAPLK
AVEEYRLVGKGAARVDIPGKATGELTYVHDMRVPGMLHGRVIRPPYAGLDCGEFVGNSLL
SVDESSIAHIPGVVAVVVIRDFVGVVALREEQAIKAAADLQVHWKAWNNALPDMSDVEQA
IRDNPRVRRTVLDQGDVDAALANASQRMPRTYLWPYQMHGSIGPSCGVADYSPSGSRVWS
GSQNPHLLRADLAWLLECEETLIDVIRMEAAGCYGRNCADDVCADALLLSRAVGKPVRVQ
LTREQEHLWEPKGTAQLMDVDGGLNADGSIAAYDFETSYPSNGAPTLALLLTGRVEPVAA
MFEMGDRTSIPPYDINNMRVTINDMAPIVRASWMRGVSALPNTFAHESYIDELAFAAGVD
PVEYRLRYLKDERAIDLVKSTAERANWAPRTAPMQTANEDHLLRGRGFAYARYIHSKFPG
FGAAWAAWVADVAIDKQTGDVSVTRVVIGHDSGMMINPAGVQHQIHGNVIQSTSRVLKER
VTFEESTVASKEWGGYPILTFPDVPQIDVMMMPRQDQPPMGAGESASVPSAAAIANAIYD
ATGIRFRELPITPERVLAALNAATLGEPAKSPGKRRKWWFGALFATLGAVLATAWPFQAE
IAPIAPPSAGTWSKATLERGRLLAAVGDCAVCHTAPGGATHAGGLAMETPFGTLYSSNIT
PDVKTGIGAWSYPAFERAMRDGIGRDGRNLYPAFPYTAFRNITDADMQALYAYLMSQAPV
SQAPTPNAMRFPFNIRPLMAGWNVLNVRRGEITPQPERGEQWNRGNYLVNGLGHCAACHS
PRNVMGAEKGGKAFLAGGMVDGWEALALNGLSKSPTPWTEEHLFAYLSTGYSDAHGVAAG
PMGPVVSELSKLPKADIRAMAVYLASLNGNATADVPVAAAVTVPNPNGRRVFEGSCKACH
ADGLGPKLFGVSPSLATNTNVHSDQPDNLIKVILQGISNPATRDLGYMPGFKDSLSDSQV
ADLAAYLRGQFAPNAPQWKGVEQKVAHLRANPGTH