Protein Info for PFLU_RS13540 in Pseudomonas fluorescens SBW25

Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00275: EPSP_synthase
  • amino acids 13 to 409 (397 residues), 302.7 bits, E=1.9e-94

Best Hits

Swiss-Prot: 69% identical to AROA_PSE14: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to pfs:PFLU2774)

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.19

Use Curated BLAST to search for 2.5.1.19

Sequence Analysis Tools

See C3KAY1 at UniProt or InterPro

Protein Sequence (418 amino acids)

>PFLU_RS13540 3-phosphoshikimate 1-carboxyvinyltransferase (Pseudomonas fluorescens SBW25)
MSSQKTVTVTPPNAPLNGKVAPPGSKSITNRALLLAALAKGTSRLSGALKSDDTRHMSVA
LRQMGVTIDEPDDTTFVVTGQGKLQLPPQPLFLGNAGTAMRFLTAAVATVEGTVVLDGDD
YMQKRPIGPLLETLGQNGIQVDSPTGCPPVTVHGVGKIKAKRFEIDGGLSSQYVSALLML
AACGEAPIEVALTGKDIGARGYVDLTLDCMRAFGAQVEAVNDTTWRVAPGGYTAHDYLIE
PDASAATYLWAAEVLTGGRIDIGVAAQDFTQPDAKAQAVIAQFPNMQATVVGSQMQDAIP
TLAVLAAFNNTPVRFTELANLRVKECDRVQALHDGLNEIRPGLATIEGDDLLVASDPALA
GTSCTALIDTHADHRIAMCFALAGLKVSGVKIQDPDCVGKTYPEYWNALGSLGVQLTY