Protein Info for PFLU_RS13475 in Pseudomonas fluorescens SBW25

Annotation: alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 PF11896: GlgE_dom_N_S" amino acids 22 to 204 (183 residues), 170 bits, see alignment E=9.9e-54 PF00128: Alpha-amylase" amino acids 228 to 334 (107 residues), 28.5 bits, see alignment E=1.8e-10 PF21702: GLGE_C" amino acids 576 to 652 (77 residues), 66.7 bits, see alignment E=2.6e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2761)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAW8 at UniProt or InterPro

Protein Sequence (662 amino acids)

>PFLU_RS13475 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase (Pseudomonas fluorescens SBW25)
MTAETQVPGTSILPLSQALLLPRIAIESTTPVIDGGEFAVKAVVGQRVNVTSKVFADGHD
KLAVLIRWRPLQDESWHSVVMTDVGNNGWEGAFTVTQQGPHEYCIEAWIDTFASFCYELR
KKHEAGVPVSLELQEGRSLVLQAAERSDNELRDRLMLLHHELSGLLETEQVAQFLHEDSA
HLMTQADHRAYLSISTVYPIDVEREAALFASWYELFPRSITDDPARHGTFNDVHSRLSMI
HDMGFDVLYFPPIHPIGRSHRKGPNNSLTAGPDDPGSPYAIGSEDGGHEAIHPQLGSRDD
FRRLVQAAADHGLEIALDFAIQCSQDHPWLKEHPGWFNWRPDGTIKYAENPPKKYQDIVN
VDFYAADAIPGLWTELRDVVVGWVEEGVKTFRVDNPHTKPLPFWQWLISDVRSKYPEVIF
LAEAFTTPAMMARLGKVGYSQSYTYFTWRNTKAELSEYLTQLNQSPWRECYRPNFFVNTP
DINPPFLHESGRPGFLIRAALATMGSGLWGMYSGFELCEAAPVPGKEEYLNSEKYEIRPR
DFTAPGNIIAEIAQLNRIRRQNPALQTHLGLKLYNAWNDNILYFGKRSEDGSNFILVAVN
LDPYNAQEANFELPLWELGLPDDAETQGEDLMNGHRWTWYGKHQWTRLEPQMPFGIWRIT
TP