Protein Info for PFLU_RS13390 in Pseudomonas fluorescens SBW25

Annotation: sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 PF00005: ABC_tran" amino acids 20 to 161 (142 residues), 118.2 bits, see alignment E=8.9e-38 PF17912: OB_MalK" amino acids 235 to 288 (54 residues), 37.8 bits, see alignment 6e-13 PF08402: TOBE_2" amino acids 281 to 356 (76 residues), 42.1 bits, see alignment E=1.5e-14

Best Hits

Swiss-Prot: 58% identical to MALK_YERPN: Maltose/maltodextrin import ATP-binding protein MalK (malK) from Yersinia pestis bv. Antiqua (strain Nepal516)

KEGG orthology group: K10230, sorbitol/mannitol transport system ATP-binding protein (inferred from 100% identity to pfs:PFLU2742)

MetaCyc: 85% identical to polyol ABC-type transporterATP-binding component MtlK (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, ATP-binding component" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAV0 at UniProt or InterPro

Protein Sequence (371 amino acids)

>PFLU_RS13390 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC (Pseudomonas fluorescens SBW25)
MANLKIKNLQKGFEGFSIIKGIDLEVNDKEFVVFVGPSGCGKSTLLRLIAGLEEVSEGTI
ELDGRDITEVTPAKRDLAMVFQTYALYPHMSVRKNMSFALDLAGVDKKLVESKVSEAARI
LELGPLLERKPKQLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQMRLELSRLH
KELQATMIYVTHDQVEAMTLADKVVVLNSGRIEQVGSPLELYHQPANLFVAGFLGTPKMG
FLKGKVTRVEGQGCEVQLDAGTTISLPLSGATLSVGSAVTLGIRPEHLEIADASGSVAQN
TLTVTADVGERLGSDTFCHVITATGEPLTMRIRGDMASQYGETLHLHLDPAHCHLFDTDG
VAVARPLRAAA