Protein Info for PFLU_RS13345 in Pseudomonas fluorescens SBW25

Annotation: type I methionyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00500: methionine aminopeptidase, type I
  • amino acids 2 to 247 (246 residues), 306.3 bits, E=8e-96
• PF00557: Peptidase_M24
  • amino acids 10 to 239 (230 residues), 170.4 bits, E=2.3e-54

Best Hits

Swiss-Prot: 51% identical to MAP1_ECOL6: Methionine aminopeptidase (map) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01265, methionyl aminopeptidase [EC: 3.4.11.18] (inferred from 100% identity to pfs:PFLU2733)

MetaCyc: 51% identical to methionine aminopeptidase (Escherichia coli K-12 substr. MG1655)
Methionyl aminopeptidase. [EC: 3.4.11.18]

Predicted SEED Role

"Methionine aminopeptidase (EC 3.4.11.18)" (EC 3.4.11.18)

MetaCyc Pathways

• Arg/N-end rule pathway (eukaryotic) (8/14 steps found)
• Ac/N-end rule pathway (6/21 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.18

Use Curated BLAST to search for 3.4.11.18

Sequence Analysis Tools

See C3KAU2 at UniProt or InterPro

Protein Sequence (253 amino acids)

>PFLU_RS13345 type I methionyl aminopeptidase (Pseudomonas fluorescens SBW25)
MIKTAAQLAVMRESGRLLAQVFTMLDGFVAAGRSTLELDSAVEAFIRHDLKARPASLGQY
DYPYSINTSINEVVCHGMPSAKEILKDGDIINIDITLEKGGYIADASKMYMIGTVAPKAR
RLVEMTFEAMWAGIRQVKPGARLGDIGHAIQSHAQANGYSVVREYCGHGIGRQMHEEPQV
LHFGRPGTGLELREGMVFTVEPMLNQGSAKVRSLKDGWTVVTKDNSLSAQWEHTVAVTAD
GFEVLTLQVQTGE