Protein Info for PFLU_RS13155 in Pseudomonas fluorescens SBW25

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 PF20161: VpsR" amino acids 6 to 115 (110 residues), 31.3 bits, see alignment E=3.9e-11 PF14532: Sigma54_activ_2" amino acids 139 to 307 (169 residues), 77.7 bits, see alignment E=2.8e-25 PF00158: Sigma54_activat" amino acids 139 to 304 (166 residues), 245.6 bits, see alignment E=5.5e-77 PF07728: AAA_5" amino acids 161 to 278 (118 residues), 25.7 bits, see alignment E=2.6e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2695)

Predicted SEED Role

"Sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9V0 at UniProt or InterPro

Protein Sequence (441 amino acids)

>PFLU_RS13155 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25)
MGGPPVQRRLLVVDPCDDCHGLLPGLRTAGWAVDSCTLEAVGDRSCDVGLLRLQPYHLER
PEAVKELIGRSGTEWIAVLSQDVLRLQNVGDFVCEWFFDFHTLPFDVARVQVTLGRAFGM
ARLRGKGQTPVDEPEHELLGDSRPIRELRKLLSKLAPTESPVLIRGDSGTGKELVAKTLH
RQSQRHAKPFVAINCGAIPEHLIQSELFGHEKGAFTGAHQRKVGRIEAANGGTLFLDEIG
DLPMELQANLLRFLQEKQIERVGGSQPIPVDVRVLAATHVDLEAAVEKGTFREDLYYRLN
VLQVVTAPLRERHGDVAMLANHFSRFYSQETGRRPRSFSDDALIAMGEHAWPGNVRELAN
RVRRGLVLAEGRQIEAVDLGLQGRQAISPPMATLEDYKHRAERQALCDVLNRHSDNLSVA
ARVLGVSRPTFYRLLHKHQIR