Protein Info for PFLU_RS13145 in Pseudomonas fluorescens SBW25

Annotation: Cof-type HAD-IIB family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 PF08282: Hydrolase_3" amino acids 12 to 266 (255 residues), 195.9 bits, see alignment E=1.6e-61 TIGR00099: Cof-like hydrolase" amino acids 12 to 266 (255 residues), 170.8 bits, see alignment E=4.1e-54 TIGR01484: HAD hydrolase, family IIB" amino acids 12 to 239 (228 residues), 92 bits, see alignment E=5.7e-30 PF05116: S6PP" amino acids 158 to 246 (89 residues), 40.3 bits, see alignment E=4.3e-14

Best Hits

KEGG orthology group: K07024, (no description) (inferred from 100% identity to pfs:PFLU2693)

Predicted SEED Role

"HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9U8 at UniProt or InterPro

Protein Sequence (274 amino acids)

>PFLU_RS13145 Cof-type HAD-IIB family hydrolase (Pseudomonas fluorescens SBW25)
MSDAAIHPIRFILSDVDGTLLHPDHSLSQRTADAVRALREAGVLFSLASGRPPKAMLHLI
ETFGIDVPVAGFNGGTLINPDGSILVAHHLPAEAALVTLALFSAEPEVEVWVFADGDWLR
RDPSGPMEPREANGLGYGPVVVESFEPYLDRVDKIVAASHNTQLLVELEARLQPKVQGLA
QVSRSQPVYLDVTAMLANKGEALKTLAAHLGVPMEQTAAIGDGGNDPAMFQVAGLSIAMG
QAEETVKRQASVVTGSNIEDGAAEAIERFILAAP